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Objective@#To optimize the ion selective electrode method of measuring urinary fluorine in WS/T 30-1996.@*Methods@#The volume of 5 mol/L NaOH solution was fixed to confect the TISAB buffer solution. The samples were prepared by mixing 5 ml urine and equal volume of TISAB solution. Fluorine electrode and calomel electrode was used to test. When the potential value changed less than ±0.5 mV in one minute, it could be recorded. The standard curve method was selected as a quantitive method.@*Results@#The linear range of this method was 0.07~50.0 mg/L, E=238.96-57.50lgC, r=0.999 8. The LOD and LOQ were 0.02 mg/L and 0.07 mg/L (with 5 ml urine) , respectively. The RSD of intra -and inter-batch precision were 0.80%~2.82% and 2.17%~2.86%, respectively. The recovery rate was 102%~107%. The urine sample could be preserved stably for 14 days at room temperature, and for 30 days at 4 ℃ and -20 ℃.@*Conclusion@#In this method, the preparation process of TISAB buffer solution was simplified, and the linear range was expanded. It could meet the needs of occupational population detection.
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Objective@#Through inter-laboratory comparison to analysis and evaluation of the detection capacity of arsenic in the urine. @*Methods@#Urine arsenic samples were prepared with normal human urine as matrix. The homogeneity of the samples was investigated and the results were statistically analyzed by single factor analysis of variance. 2 samples were issued to each participant laboratory. A robust statistical four-point distance method was used to calculate the results submitted by each participant laboratory and the test capability of the laboratory was assessed by the z-score method. By means of method experiments and records, the reasons of dissatisfaction and the influencing factors of the results were discussed. @*Results@#The statistic of the homogeneity of urine arsenic samples was less than the critical value (P>0.05) , which showed that the arsenic in the sample was homogeneous. The satisfactory rate of comparison between 36 laboratories was 86.1%. The main reasons for dissatisfaction were the testing conditions and the quality control measures. The selection of sample pretreatment, acidity control and hydrogenation conditions was the main influencing factor for the determination of urinary arsenic by atomic fluorescence spectrometry. @*Conclusion@#The level of urinary arsenic detection in the occupational health laboratories was generally better and a few laboratories need to be improved ability of detection. It was very important to control the test conditions reasonably and strengthen the quality control measures to improve the accuracy of urine arsenic detection.
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Objective@#To analyze the results obtained from the proficiency testing program for determination of bovine blood lead and urinary cadmium, so as to evaluate the proficiency of the laboratories, and improve the detection and quality control capability of occapational health laboratory.@*Methods@#Prepared 3 levels of lead samples with bovine blood as base material, taking Technical Norm of Primary Reference Material as Operating standard, 60, 80, 180 μg/L were used as the standard concentration. All samples were made for 200 bottles. Normal urine were used to prepared 3 levels of cadmium samples with 5.0, 7.0, 10.0 μg/L as standard concentration, then 3 level of samples were made for 200 bottles respectively. Blood lead and urinary cadmium samples were sent to each laboratory in the form of sample pairs. One-way ANOVA was used to investigate the homogeneity. The results of each laboratory were statistically analyzed by robust statistical method, and the detection ability of the laboratory was evaluated by the Z ratio score method. By consulting the test reports and original records of each participating laboratory, the reasons for the unsatisfactory results were analyzed.@*Results@#The statistical F of the uniformity of blood lead 1.42, 1.37 and 2.85, F<3.13, P were 0.294, 0.314 and 0.059, respectively in 31 labs. There is no statistical significance in the difference of blood lead (P>0.05). And urine cadmium was less than the critical value (P>0.05). In all, 31 laboratories participated in the test of blood lead, and 28 loboratories paticipated in urine cadmium verification. The statistical F of the uniformity of urinary cadmium 1.90, 1.35 and 1.19, F<3.13. The homogeneity of the samples were in accordance with the requirements of proficiency testing program. The satisfactory rate of the results for blood lead was 84% (26/31) , and the satisfactory rate of urinary cadmium was 93% (26/28) . In the test of blood lead, 21 of the 31 laboratories were controlled by the standard material method. Totally, 20 of them get an satisfactory level, the rate was 95%.@*Conclusion@#Most of the laboratory test results were satisfactory, which showed that the detection capability for blood lead and urine cadmium was good. The main causes of unsatisfactory results were detection methods, tools of quality control ortraceability standards.
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Objective@#To establish a method to determine fluorine ion in human urine by ion chromatography method.@*Methods@#The samples were centrifuged, diluted and processed by C18 solid-phase column, potassium hydroxide as mobile phase, gradient elution, separated by AS15, and determined by anion conductivity detector.@*Results@#The linear correlation coefficient of fluoride ion in the range of 0.02-10.00 mg/L was more than 0.999. The lowest detection limit of urinary fluoride was 0.06 mg/L (in 3 ml urine) . The recoveries of the method were 96.9%-99.5%, the inter batch precision range was 0.40%-2.89%. The intra batch precision was from 1.01 to 7.23%. Fluoride samples could be stored for 7 days at room temperature and 4 ℃, and be stored for 14 days at -20 ℃.@*Conclusion@#Determination of fluoride in urine by ion chromatography, the method could meet the requirement in GBZ/T 210.5-2008.
ABSTRACT
Chronic prostatitis has similarity with pyocutaneous disease in pathogenesis oftraditional Chinese medicine and western medicine, pathology ofWestern medicine and signs and symptoms ofdisease, we got quiet cutative effect in treating chronic prostatitis from treatment theory ofpyocutaneous Disease.Based on theory ofdiagnosis and treatment ofpyocutaneous disease, we should synthesize differential diagnosis and treatment according to different stages, conform three methods, focus on eliminating method and strengthening vital qi, pay attention to two-level multi-object, involving macro-level and microlevel, and combination oftraditional and western medicine.The common used ancient prescriptions are Wuwei Xiaodu Yin, Gouju Decoction, Chaihu Shengshi Decoction.The common used empitical formula are Qinshi Simiao San, Qianliexian Xiaodu Fang, Qianlixian Tuodu Fang, Qianliexian Fuzheng Fang, Tongjing Huoxue Tang, except for these formula herbs, there are other common used herbs, such as processed zaoci, baijiangcao, hongteng, lianqiao etc.According to sydrome differentiation at stages ofbeginning, middle, the later, the chronic prostatitis can be divided into acute episode and chronic episode.In clinics, we should pay attention to taking care ofpatient's splee and stomach, increasing compliance ofpatient, inducing details ofdiet and living which will enhance clinical effective .