Response of green synthesized drug blended silver nanoparticles against periodontal disease triggering pathogenic microbiota

Objective: Evidences for microorganisms to predominate periodontal disease, alteration of human pathogenshifted microbiota by Erythrina fusca, and antimicrobial response potentiation by ecofriendly silvernanoparticles (AgNPs) intended present study to perform green synthesis of AgNPs and evaluate againstperiodontal disease triggering pathogenic microbiota.Material and Methods: Present study involved green synthesis of AgNPs using Erythrina fusca leavesaqueous extract (EFLAE), followed by optimization, characterization, stability, and evaluation of antimicrobialpotential of biosynthetic AgNPs against periodontitis causing pathogenic human microflora (Pseudomonasaeruginosa, Escherichia coli, Streptococcus pyogenes, and Staphylococcus aureus).Results: The AgNPs green synthesis success was based on brown coloration and surface plasmon resonancesignal at 433 nm. UV-Visible spectrometry driven optimization determined 5 mM AgNO3 concentration, 1:9EFLAE and AgNO3 volumetric ratio, pH 7, 60°C temperature, and 2 hours’ time as parametric requirementsfor AgNPs biosynthesis. Stability studies revealed signal appearance between 415 and 424 nm supportingAgNPs stability. Characterization studies recognized shifted and broadened Fourier transformed infrared bandsof AgNPs revealing silver capping by biochemical moieties of EFLAE; AgNPs size below 32 nm in fieldemission scanning electron microscopic micrograph; X-ray diffraction signals at 38.95, 44.97, 64.92, and78.97 representing 111, 200, 220, and 311 AgNPs silver cubic structure planes; and elemental silver 83.66%,carbon 11.87%, and oxygen 4.47% in energy-dispersive X-ray spectrum. Optimized and characterized biogenicAgNPs, when evaluated against periodontal disease-causing pathogenic microflora using well diffusion method,exhibited maximum inhibitory zone (in mm) against Bacillus cereus (13 and 18), followed by P. aeruginosa (11and 19), E. coli (10 and 18), and S. pyogenes (9 and 15) in 50 and 100 µg/ml administered dose.Conclusion: Present study concludes that biogenic AgNPs synthesized using EFLAE possess high inhibitionpotential against periodontitis triggering pathogens (E. coli, B. cereus, S. pyogenes, and P. aeruginosa) ofmicrobiota, and recommends EFLAE as potential source for AgNPs green synthesis

Phytochemical constituents and pharmacological activities of Lagerstroemia floribunda Jack. (Kedah bungor): A Review.

This is the first report on review of Lagerstroemia species which includes the detailed description on phytochemistry and its pharmacological research. Despite being rich in important phytochemicals and having possible medicinal value there is no enough information available on this plant. Lagerstroemia floribunda Jack also known as Thai crape myrtle and kedah bungor, is a species of flowering plant in the Lythraceae family. This delightful ornamental plant is native to subtropical and tropical South-East Asia, from southern China to Myanmar, Thailand, Cambodia, Indo-China and Peninsular Malaysia. The phytoconstituents of Lagerstroemia floribunda includes 23-hydroxyursoIic acid, alphitolic acid, ursolic acid, dihydro-β-cyclopyrethrosin, sesamin, β -sitosterol, clauslactone-K, betulinic acid, lingueresinol, ent-isolariciresinol. Some of the researches that are being carried out on this plant include total phenolic contents and antioxidative activity of its flowers. This review will help to provide detailed information on recent researches done on this plant.