ABSTRACT
Objective To observe the effects of renin - angiotensin system ( RAS ) and steroid hormones onpregnancy in in vitro fertilization and embryo transfer(IVF-ET). Methods .53 tubal infertility women underwent IV F-ET under control of ovarian hyperstimulation with GnRHa/FSH/hMG/hCG. Plasma total renin, serum estradiol(E2),progesterone(P) and testosterone(T) were measured on the day of injection of injection of hCG and 36h after hCG( before getting the oocytes). ResultsPlasma total renin level on the day of hCG in pregnant group was higher than that in nonpregnant group(P<0.05), E2 and P lower than that in nonpregnant group(P<0.05, P<0.01). Plasma total renin and E2 levels 36h after hCG in pregnant group were lower than that in nonpregnant group( P<0.05). There was no difference in other hormones between the two groups. ConclusionThe high concentration of total renin on the day of hCG injection and its low concentration 36h after hCG administration are probably conducive to pregnancy.
ABSTRACT
Objective To investigate the expression of insulin like growth factor Ⅱ(IGF Ⅱ) and its type Ⅰ receptor (IGF ⅠR) and type Ⅱ receptor (IGF ⅡR) in the endometrium of women with unexplained infertility and their relation to steroid levels Methods In situ hybridization method was utilized to detect the location of IGF Ⅱ, IGF ⅠR and IGF ⅡR mRNA Reverse transcription polymerase chain reaction (RT PCR) method was employed to detect quantitatively IGF Ⅱ, IGF ⅠR , and IGF ⅡR mRNA expression levels in the endometrium during mid luteal phase Serum concentrations of estradiol and progesterone were measured by radioimmunoassay Thirty eight patients with unexplained infertility were included as study group, and 20 patients with men factor infertility or normal volunteer women were selected as control group Results IGF Ⅱand IGF ⅡR mRNA were diffusely distributed in the cytoplasm of stromal cells and IGF ⅠR mRNA was observed in the cytoplasm of epithelial cells Endometrial IGF Ⅱ,IGF ⅠR and IGF ⅡR mRNA expression during mid luteal phase in study group were significantly lower than those in the control group respectively (0 71 ?0 34 vs 0 96?0 34, P
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Objective To investigate the expression of matrix metalloproteinases 9 (MMP 9) and tissue inhibitor of metalloproteinases 1(TIMP 1) mRNA in the endometrium during mid luteal phase in women with unexplained infertility and its steroidal regulation Methods In situ hybridization method was utilized to detect the location of MMP 9 and TIMP 1 mRNA. Reverse transcription polymerase chain reaction (RT PCR) method was utilized to detect and quantitate MMP 9 and TIMP 1 mRNA expression levels in the endometrium during mid luteal phase. Serum concentration of estradiol (E 2) and progesterone (P) were measured by radioimmunoassay. Thirty eight patients with unexplained infertility were included as study group, and 20 women with male factor infertility or normal volunteers were selected as control group. Results MMP 9 and TIMP 1 mRNA were diffusely distributed in the cytoplasm and neuclei of glandular and stromal cells. Their staining in control group were more abundant than those in study group. Endometrial MMP 9 and TIMP 1 mRNA expression during mid luteal phase showed by RT PCR in the study group were significantly lower than those in the control group (0.42?0.19 Vs 0.57?0.19, P
ABSTRACT
Objective To analyze the factors influencing the outcome in a frozen thawed embryo transfer (FTET)program Methods Sixty FTET cycles performed in 53 cases from September 1997 to May 2000 were analyzed retrospectively The related parameters were compared between the conceived and non conceived cycles Results One hundred and seventy eight out of 252 thawed embryos were survival Embryo transfers were undergone in 57 cycles (53 patients), resulting in 17 pregnancies (30% per transfer or 28% per thawed cycle) There were no significant difference between conceived and non conceived cycles in terms of the controlled ovarian hyperstimulation protocols, numbers of developed follicle and oocyte retrieved, fertilization rate, numbers of frozen embryo and the luteinizing hormone, estradiol, progesterone levels on human chorionic gonadotropin triggering day The percentage of good quality embryo before freezing and after thawing and survival thawed embryos were higher in conceived cycles than those in non conceived cycles (83 8% Vs 60 7%, 76 8% Vs 50 0%, 82 4% Vs 66 3%, respectively P