ABSTRACT
Objective: To determine the role of glutathione S-transferase [GST] isoenzyme polymorphisms as susceptibility factors in patients with psoriasis in a Turkish cohort
Subjects and Methods:In this case-control study, 105 patients with plaque-type psoriasis and 102 healthy controls were recruited from the dermatology outpatient clinics of two university hospitals. Genomic DNA was extracted from whole blood using a DZ DNA isolation kit. Multiplex PCR was used to determine GSTM1 and GSTT1 polymorphisms in the isolated DNAs
Results:Of the 150 patients with psoriasis, 83 [79%] were identified with the GSTT1 genotype and 22 [21%] with the null genotype. Of the 102 patients in the control group, 69 [67.6%] subjects were identified with the GSTT1 genotype and 33 [32.4%] with the null genotype. There was no significant difference between the patient and control groups [p = 0.063]. Regarding the GSTM1 polymorphism, 54 [51.4%] patients were identified with this genotype and 51 [48.6%] with the null genotype; in the control group, 50 [49%] were identified with this genotype and 52 [51%] with the null geno- type. Again there was no statistically significant difference between the groups [p = 0.957]
Conclusion:In this Turkish cohort of patients with psoriasis, neither GSTT1 nor GSTM1 polymorphisms were associated with disease susceptibility. Larger studies with a wider range of GST isoenzyme are needed