ABSTRACT
Some essential oils and their main components have antibacterial activity and used as an antiseptics. Respitol-B [Barijessence, Kashan, Iran] which is similar to Mentofin, an imported antimicrobial agents, contains menthol and eucalyptus oil. Both products are used as disinfectant in poultry farms following vaccination of broilers and pullets. In this study, the antimicrobial activity of Respitol-B and Mentofin, against bacteria, fungi and yeast was investigated. The antimicrobial activities of eucalyptus oil and mentho was also tested separately using disk diffusion test and macrobroth dilution assay. The gram-positive bacteria, yeast and fungi showed more susceptibility to Respitol-B than the gram negative bacteria. Antimicrobial activity of Respitol-B was the same as mentofin. Menthol is more potent than the eucalyptus oil. It enhanced the antimicrobial activity of Respitol-B. However P. aeruginosa, E. coli and S. typhi showed resistance to this compound. Eucalyptus oil had antimicrobial effect against V. chlerae, A. flavus, and S. aureus but had no effect on other tested microorganism. Respitol-B, a compound formulated by Barij Essence shows the same an antiseptic property as Mentofin and can be used for sanitary protocols in poultry farms