ABSTRACT
This study deals with the macroscopic, microscopic pathology, associated with the immunopathological and virological diagnosis of these field outbreaks. The results showed that the viruses caused variable degrees of erosions and ulceration throughout the different parts of upper and lower digestive tract. Also, hepatitis, bronchopneumonia, trachietis, glomerulonephritis and severe mycoarditis were seen. These pathological lesions were illustrated and fully discussed. Vesicular fluid and the suspensions prepared from vesicular epithelium of diseased animals were used as antigen in complement fixation test against antisera to each of the seven types of FMD virus. It was showed that the virus was 0 serotype. By application of agar gel immunodiffusion test, the thyroid and mesenteric lymph nodes gave clear precipitating b and s against the st and ard BVD antiserum. Results of the immunofluorescence showed that samples which gave positive AGID test gave strong specific fluorescent reaction. The previously mentioned results were confirmed by applying immunoperoxidase test on the tissue against FMD type 0 antiserum and BVD antiserum. The immunoperoxidase reaction revealed dense brown precipitate in the cells lining the tongue, intestine, rumen, liver, kidney, spleen and lymph nodes, against BVD and FMD viruses