Immunological studies of the Egyptian water buffalo [bos bubalis]: preparation of immunoglobulins from buffalo serum

Three classes of buffalo immunoglobulins were chemically precipitated as crude materials. Using column chromatography, they were obtained in pure fractions and were designated as: IgM, IgG I and IgA. The IgG was clearly separated into 2 sub classes: IgGI and IgG2. The purity of the prepared fractions was tested and proved by Ouchterlony and Immunoelectrophoresis techniques against polyvalent and monospecific anti-cow IgM, IgG and IgA antisera