1.
Pakistan Journal of Biochemistry. 1988; 21 (1-2): 37-41
in English
| IMEMR
| ID: emr-11524
ABSTRACT
A highly sensitive radioimmunoassay for rat pancreatic kallikrein with a detectable amount of 0.2 ng/ml has been developed. The assay uses an antiserum against purified rat submandibular kallikrein in a final dilution of 1:240,000. Optimum conditions of incubation period [20 hours], temperature [25°C] and minimal amount of labelled antigen [1/650 dilution] have been determined. The reproductibility of the radioimmunoassay has been demonstrated by applying linear regression analysis