ABSTRACT
A survey of several plant seeds which may possess phytohemagglutinating properties indispensable in blood studies and tissue cultures demonstrated that five plant seeds have this property, namely: Red Kidney beans, White Kidney Beans, Sigarilyas, Bataw and Soy Bean (local variety)The first two agglutinate blood type A, B, AB and O while sigarilyas A, B and AB; Bataw, A, B and AB while the local soy bean agglutinates only type B and ABBy column chromatography in Ve/Vo studies, RKB, WKB and Sigarilyas had molecular weights of 61,000, 62,000 and 60,000, respectively. Estimated MWs of PHA from RKB, WKB and Sigarilyas compared with the molecular weights of known standard compounds by TLG show RKB, WKB and Sigarilyas had MWs of 62,000, 57,000 and 40,000, respectivelyJaffes Method of Purification has been found most suitable for sigarilyas PHA extractionA comparison of the 1/Rf values of ovalbumin for both column chromatography and Thin Layer Gel Filtration shows that the calibration curves are reliable. However, based on the results of the molecular weight determination or the PHA from the samples, the MWs obtained by column chromatography and TLG differ, the separation by the latter seem more distinct. (Summary)
ABSTRACT
Litrature survey appears to show that a diet rich in nitriloside may ba an answer to preventive medicineA survey of Philippine plant and other plant products for nitrilosides content was carried out using qualitative and quantitative tests. Results show that among the plants used in the Filipino diet, cassava, patani, chesa , ginger and singkamas were particularly rich in nitrilosides. Fresh sorghum grains were also found to be an excellent source of nitrilosideExperimental study of the effect of nitriloside on health and nutrition was made on rabbits and the results showed that the experimental animals fed raw cassava bark and leaves gained weight and improved their blood picture as compared with the control animals. The preliminary study on culture of cancer cells showed significant inhibition by nitriloside on the dehydrogenase using methylene blue as the inidicatorStudies are now undergoing to extract the nitriloside, known as linamarin from cassava. Can linamarin show a more significant improvement in the blood picture of experimental animals?. Will linamarin show any inhibition on the culture of P-388 leukemic cells? This we hope to find out. (Summary)