Synthesis of certain pyrazolol [3,4-d] pyrimidine derivatives of potential anti-inflammatory activity

The synthesis of twenty nine novel derivatives of pyrazolol [3,4-d] pyrimidines as non acidic nonsteroidal anti-inflammatory drugs has been achieved via reaction of 4-chloro-l-phenylpyrazolo [3,4-d] pyrimidine with different substituents including 4-amino-3-methylphenol, 4-phenylene diamine and 4-acetamidophenol. The anti-inflammatory activity of thirteen representative compounds have been screened compared to indomethacin as a reference drug. The results revealed that all the tested compounds showed anti-inflammatory activity with the exception of 10a.