Estimation of bioactive potential of culturable bacterial endophytes from Coleus

Endophytic microflora is source of several bioactive compounds. Endophytes isolated from Coleus species are yet to be fully explored for their bioactive potential. In this study, bacterial endophytes were isolated from three different species of Coleus. Isolated endophytes were characterized by using Gram staining and by sequencing 16S rRNA region. Further, solvents with different polarities were used to prepare extracts which were used for assessment of different bio-activities including in vitro cytotoxicity, anti-microbial and anti-oxidant activities. Also, the pure endophytic bacterial cultures were evaluated for their antiphytopathogen potential as well as indole-3-acetic acid (IAA) and protease production. Advanced studies on the endophytes with promising activities may lead to the isolation of novel natural products for drugs as well as in industrial and agricultural applications.

Isolation, identification and bioactive potential of bacterial endophytes from Coleus

Coleus (Lamiaceae) is a large and widespread genus comprising of species with diverse ethnobotanical uses. In the present study, bacterial endophytes were isolated from Coleus forskohlii and Coleus aromaticus. Endophytes are the microorganisms which reside within the plants without showing any harmful effect on its host. Diverse types of endophytes live symbiotically within almost all plants and in turn help the plant in a number of ways such as imparting resistance against biotic and abiotic stresses, producing compounds involved in attraction of pollinators, inducing the plant defense mechanisms, etc. The bacterial endophytes isolated in this study, were characterized by microscopic examination (using gram staining) and molecularly identified by sequencing the 16S rRNA. Extracts were prepared from endophytic biomass using solvents of different polarities (methanol, ethyl acetate and butanol) and were screened for their bioactive potential (in vitro cytotoxicity anti-microbial, and anti-oxidant activity). Scale-up of endophytes showing promising results is under process, which will help in isolation of pure compounds.

Influence of symmetric and asymmetric alterations of maxillary canine gingival margin on the perception of smile esthetics among orthodontists, dentists, and laypersons.

Introduction: Esthetics is one of the major concerns among people seeking orthodontic treatment, and its perception varies from person to person. Our objective was to determine the differences in the perception of smile esthetics among orthodontists, general dentists, and laypersons with respect to alteration in the maxillary canine gingival margin in close‑up smile analyses. Materials and Methods: Close‑up photograph of an ideal Indian female smile was selected. The images were digitally altered to create symmetrical images with the gingival margin levels of maxillary canine matching the central incisors. Twelve alterations were created in the gingival margin of the canine with discrepancies of 1, 2, and 3 mm in relation to the most superior point on the labial gingival margin of patient’s central incisor and divided under four groups. Finally, close‑up images of the smile were assessed by orthodontist, general dentist, and layperson who indicated the level of attractiveness of each smile on a visual analogue score. The data collected were then statistically analyzed by one‑way analysis of variance followed by Newman–Keul’s range test. Results: Most evaluators considered all the three components, i.e., lips, teeth, and gingiva while assessing the smile’s attractiveness. Statistically significant differences were observed between different evaluator groups when considering bilateral increase in crown height by 2 and 3 mm. The perceptions of asymmetries in the gingival margin levels of the maxillary canines were 1.0 mm for orthodontists and 2.0 mm for laypersons. Conclusion: The result of this study suggested that the orthodontists were more critical than dentist and layperson in evaluating smile esthetics.

Perception of smile esthetics among Indian dental professionals and laypersons.

Introduction : Patients' perceptions and expectations regarding their appearance play a significant role in treatment planning. The purpose of this study was to compare the perceptions of orthodontists, general dentists, and laypersons regarding smile esthetics after symmetrical and asymmetrical alterations in anterior teeth and their supporting tissues. Materials and Methods: Alterations were made in the crown length, crown width, midline diastema, and gingiva-to-lip relationship of the maxillary anterior teeth in the close-up photograph of a woman's smile. The attractiveness of the smile in the original image and in each of the modified images were assessed by orthodontists (n=40), dentists (n=40), and laypersons (n=40) and scored using a visual analog scale (VAS). The mean VAS scores were calculated for each photograph. ANOVA was used for comparisons between photographs and between groups. To determine threshold levels at which each group discriminated between esthetic and less esthetic dental features were determined by one-way ANOVA (F-test) followed by Newman-Keul's range test. Results : Orthodontists were found to be more critical when evaluating smile images compared to general dentists and laypersons. Symmetrical or asymmetrical alterations in the mesio-distal width of the lateral incisor of up to 2 mm was not perceived as unesthetic by general dentists and laypersons. Conclusion: Laypersons are more accepting of minor variations in anterior tooth size and alignment than orthodontists.