ABSTRACT
The present study was conducted on the protein extracted from the pulp that obtained by cannabis oil-pressingand loading the bioactive peptides into nanoliposomes. Physical properties of empty and loaded nanoliposemes(average particle size, polydispersity index, and zeta potential) and encapsulation efficiency were evaluated.Moreover, the effect of storage condition (fridge and ambient temperature) on the physical stability and maintainingthe encapsulation efficiency in nanoliposomes loaded with peptides was investigated. Eventually, the effect ofpeptide loading on the nanoparticle chemical structure Fourier-transform infrared (FTIR) as well as the morphologyof nanocarriers scanning electron microscopy (SEM) was evaluated. Physical properties of nanoliposomes wereaffected by the hydrolyzed type. The average particle size and polydispersity index of nanoliposomes varied from79.5 to 101.5 nm and 0.234 to 0.326, depending on the type of loaded peptides. The zeta potential of nanoliposomeswas changed from −10.32 to −15.33 mV when loaded with the peptide obtained by enzymatic hydrolysis during300 min. The efficiency of nanoliposomal encapsulation varied from 90.7 to 81.4%. Thus, peptides with the highestdegree of hydrolysis had the least encapsulation efficiency. The evaluation of physical stability and the maintenanceof encapsulation efficiency indicated the highest stability of nanoliposomes, which were stored at fridge temperature.FTIR spectroscopy in empty nanoliposomes and those loaded with peptides implied the presence of peptides inthe polar regions of phosphatidylcholine, such as the internal regions of the liposomes and the formation of ioniccomplexes between them. Conversely, SEM images of the structural and superficial properties of nanoliposomesindicated the existence of dense and compact clusters of the spherical nanoparticles with flat surfaces.
ABSTRACT
Our study aimed to develop a survey that could be used by nurses during regular cystic fibrosis [CF] clinic visits, providing clinicians with a standardized means of longitudinally assessing and monitoring symptom progression in their patients. In addition, the use of this survey would provide an opportunity for patient engagement and relationship building, thereby enhancing patient education and improving adherence to treatment. This is the first such survey designed specifically for use in Arab populations. The Cystic Fibrosis Symptom Progression Survey [CF-SPS] was developed using previously published patient reported outcomes relating to pulmonary exacerbations in CF. It contains 10 items that provide a patient-focused account of symptoms. The survey was translated into Arabic and was completed by 12 patients on 139 occasions over 22 months. The psychometric properties of the survey were evaluated, as was the relationship between the survey findings and other known clinical measures of health status in CF. The CF-SPS performs well as a psychometrically valid clinical tool, with good internal consistency as determined by Cronbach's alpha analysis. Our results suggest that the CF-SPS is able to identify significant declines in health status in line with routine clinical patient assessment [chest sounds, body mass index and admissions]. As such it is a useful tool that can support clinical decision making in the care of Arabic speaking CF patients. We recommend the CF-SPSa [Arabic version] as a valid tool for the longitudinal monitoring of symptom progression in CF in Arabic speaking populations