ABSTRACT
AIM and METHODS: A study of radioimmunoimaging was carried out on Kcnming mice - uterine cervical cancer (U14) using 99mTc labeled monoclonal Au14-1 with a modified Schwartaz method. RESULTS: The bio - distribution showed that radioactivity accumulated in tumor tissue at 12h after 99mTc - Au14- 1 injection in tail vein. The uptake by tumor was 4. 12 % ID/g at 2h and 8. 79 % ID/g at 24h respectively. The tumor/non - tumor (T/NT) radiocativity ratios for organs except kidneys were ranged from 2.02 to 6.71 at 24h post - injection. The image of tumor showed at 12h and clearer at 24h after injection. CONCLUSION: The quality of tumor image was relevant to the T/NT radioactivity ratios. It was demonstrated that 99mTc- Au14-1 has a good capability of localization for tumor.
ABSTRACT
AIM: To investigate the effect of monoclonal antibody(McAb) AU_(14-1) mediated cytotoxicity against cervical cancer U_(14) cell in vitro.METHODS: MTT colorimetric assay was applied to study the McAb AU_(14-1) mediated cytotoxicity of effector cells including splenocytes,peritoneal macrophages and LAK to U_(14) cells.RESULTS: The cytotoxicity mediated by each effector cells to the U_(14) cells treated with McAb AU_(14-1) was significantly higher than those not treated with it(P
ABSTRACT
The TD。 of mouse uterine cervical carcinoma (U_(14)) were inoculated subcutaneously in syngenic mice. After that experimental mice were administered diverse doses of monoclonal anti-U_(14) antibody (AU_(14-1)). It was found that within a 267 day interval after the tumor cell inoculations, all control animals were tumor free with tumor free survival, but 86% (6/7)and 88% (7/8)of experimental mice that had been treated with high dose AU_(14-1) showed progressive tumor growth at the inoculation site and died of systemic tumor disease. These results indicate immune enhancement of AU_(14-1) on U_(14) cells. AU_(14-1) was used to study the response of U_(14) cells to specific anti (?)dy in vitro. The results demonstrate that AU_(14-1) induces antigenic modulation of U_(14) cells, which is shown to be a loss of AU_(14-1) antibody and U_(14) antigen from the surface membrane of these cells as determined by indirect membrane immunofluorescence assay. This suggested that antigenic modulation may be proposed as a mechanism by which cervical cancer cells escape monoclonal antibody therapy.
ABSTRACT
In this article, monoclonal antibody to mouse uterine cervical carcinoma (U_(14)), AU_(14-1), was used for immunohistochemical analysis of premalignant and malignant lesions of the cervix to investigate the incidence and significance of the expression of the antigen detected by AU_(14-1) in human cervical lesions. Peroxidase-antiperoxidase (PAP) staining was performed on formalin-fixed, paraffin-embedded biopsy specimens. It was reported that positive staining with AU_(14-1) was detected in 20 of the 20 squamous cell carcinomas, 13 of the 13 adenocarcinomas, 15 of the 21 carcinomas in situ, 2 of the 24 anaplasia, and 2 of the 20 chronic cervicitis. It is suggested that the expression of the U_(14) antigen may be related to malignant transformation in the cervix.