Analysis of mdr1-1Δ mutation of MDR1 gene in the Cimarron Uruguayo dog / Análisis de la mutación mdr1-1Δ del gen MDR1 en el perro Cimarrón Uruguayo

Objective. The aim of this paper is to analyze the frequency of the mdr1-1D mutation of the MDR1 gene in a dog sample of the Uruguayan Cimarron breed with the objective of increasing the knowledge of this breed’s genome. Materials and methods. Thirty-six animals of this breed were analyzed. The MDR1 gene region, which includes the location where the mutation would be present, was amplified by PCR. Results. The mutation was not detected in any of the analyzed Uruguayan Cimarron. Conclusions. The lack of described ivermectin intoxication cases in veterinary clinic in this breed is explained by the lack of the mutation object of this study. The sequence studied in Cimarron dogs is kept compared to other breeds, except Collies and related breeds (Border Collie, Bearded Collie, Old English sheepdog).

Objetivo. El propósito de este trabajo fue analizar la frecuencia de la mutación mdr1-1D del gen MDR1 en una muestra de perros de raza Cimarrón Uruguayo con el fin de profundizar en el conocimiento del genoma del animal. Materiales y métodos. Se analizaron treinta y seis animales de la raza mencionada. La región del gen MDR1 que incluiría la mutación se amplificó por PCR. Resultados. En los perros cimarrones analizados no se detectó la mutación mencionada. Conclusiones. La ausencia de la mutación estudiada explicaría el hecho de que no se hayan descrito casos de intoxicación por ivermectina en esta raza. La secuencia en el perro Cimarrón es conservada respecto de otras razas caninas, excepto Collie y sus derivadas.

Estudios preliminares de la estructura genética del perro cimarrón uruguayo usando microsatélites / Preliminary studies of the genetic structure of cimarron uruguayo dog using microsatellite markers

Objetive. To analyze the population structure, using microsatellite markers in a sample of “Cimarron Uruguayo” dogs. Materials and methods. Thirty dogs were analyzed in different areas of Uruguay with a set of nine molecular microsatellite markers using PCR. The population structure was analyzed using the free distribution software “Structure’’. Results. According to our data, the preliminary results show that it is not possible to establish a subdivision among the animals in the sample. Conclusions. The study supports the hypothesis that the currently existing canines derive from a founding nucleus that took refuge in the Northeastern region of the country. The distribution of the breed among the different areas of Uruguay continues nowadays, so there is no isolation among the different groups of animals, and the exchange is constant.