ABSTRACT
Using cDNA microarray analysis, we previously identified a set of differentially expressed genes in primary breast tumors based on the status of estrogen and progesterone receptors. In the present study, we performed an integrated computer-assisted and manual search of potential estrogen response element (ERE) binding sites in the promoter region of these genes to characterize their potential to be regulated by estrogen receptors (ER). Publicly available databases were used to annotate the position of these genes in the genome and to extract a 5’flanking region 2 kb upstream to 2 kb downstream of the transcription start site for transcription binding site analysis. The search for EREs and other binding sites was performed using several publicly available programs. Overall, approximately 40 percent of the genes analyzed were potentially able to be regulated by estrogen via ER. In addition, 17 percent of these genes are located very close to other genes organized in a head-to-head orientation with less than 1.0 kb between their transcript units, sharing a bidirectional promoter, and could be classified as bidirectional gene pairs. Using quantitative real-time PCR, we further investigated the effects of 17β-estradiol and antiestrogens on the expression of the bidirectional gene pairs in MCF-7 breast cancer cells. Our results showed that some of these gene pairs, such as TXNDC9/EIF5B, GALNS/TRAPPC2L, and SERINC1/PKIB, are modulated by 17β-estradiol via ER in MCF-7 breast cancer cells. Here, we also characterize the promoter region of potential ER-regulated genes and provide new information on the transcriptional regulation of bidirectional gene pairs.
Subject(s)
Female , Humans , Breast Neoplasms/genetics , Estradiol/genetics , Gene Expression Regulation, Neoplastic/genetics , Receptors, Estrogen/genetics , Receptors, Progesterone/genetics , Response Elements/genetics , Breast Neoplasms/metabolism , Cell Line, Tumor , Estradiol/metabolism , Estradiol/pharmacology , Oligonucleotide Array Sequence Analysis , Real-Time Polymerase Chain Reaction , Receptors, Estrogen/metabolism , Receptors, Progesterone/metabolism , Response Elements/drug effects , Transcription Factors/genetics , Transcription, Genetic/geneticsSubject(s)
Adult , Middle Aged , Humans , Male , Body Temperature , Cold Temperature , Energy Intake , Heart Rate , Immersion , Oxygen ConsumptionSubject(s)
Pregnancy , Adult , Humans , Female , Fetal Heart , Heart Rate , Isoantigens , Fetal MonitoringABSTRACT
Os autores utilizaram prostaglandina F2 alfa na inducao do parto em 19 pacientes com idade gestacional superior a 35 semanas. Em doses adequadas, nao houve complicacoes clinicas de grande importancia, a nao ser em um caso onde acidentalmente ocorreu superdosagem.Em apenas uma vez ocorreu falta da inducao. Os recem-nascidos tiveram indice de Apgar satisfatorio e apresentaram ocorrencia nao usual de ictericia neonatal, que merece melhor investigacao
Subject(s)
Adolescent , Adult , Humans , Female , Labor, Induced , Prostaglandins FABSTRACT
Estudaram-se 20 casos de obitos maternos ocorridos no Hospital do Servidor Publico Estadual Francisco Morato de Oliveira, de Sao Paulo, no preiodo de dez anos. O coeficiente de mortalidade materna foi de 7,20/10 mil nascidos vivos, numero esse bastante favoravel quando se compara aos de varios autores nacionais e estrangeiros. A maioria dos obitos deveu-se a causas obstetricas diretas (10) seguidas por causas nao obstetricas (8) e pelas obstetricas indiretas (2). Das obstetricas diretas, o maior contingente foi consequencia de infeccoes (5), seguido das hemorragias (3) e a toxemia (2)