ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of tetrandrine on reversion of mice S180's obtained multi-drug resistance tumor cell induced by chemotherapy by PFC. And then discuss the molecular mechanism of it for the use of TCM in clinic to restrain the drug-resistant of chemotherapy, thereby improve the curative effect.</p><p><b>METHOD</b>By the methods of less dosage of chemotherapy PFC, give the mouse cisplatin 3 mg x kg(-1) i.p., once a week; CTX and 5-FU 3 mg x kg(-1) i.g. four weeks, set up the mice models of multi-drug resistance of S180 tumor cell, and then observe the P170, Fas, CD54 and apoposis by flow cytometry.</p><p><b>RESULT</b>Tetrandrine can obviously lower the express of P170 increase the express of Fas and the apoposis of drug resistant tumor cell. And at the same time it can obviously reduce the express of intercellular adhesion molecule (CD54).</p><p><b>CONCLUSION</b>Terandrine, with its adjustment of correlated biotic active matter, can intervene the occurrence of the multi-drug resistance of tumor cells induced by chemotherapy.</p>
Subject(s)
Animals , Mice , Alkaloids , Pharmacology , Antineoplastic Agents, Phytogenic , Pharmacology , Antineoplastic Combined Chemotherapy Protocols , Pharmacology , Apoptosis , Apoptosis Regulatory Proteins , Benzylisoquinolines , Pharmacology , Drug Resistance, Multiple , Drug Resistance, Neoplasm , Glycoproteins , Metabolism , Intercellular Adhesion Molecule-1 , Metabolism , Membrane Glycoproteins , Metabolism , Sarcoma 180 , Metabolism , Pathology , TNF-Related Apoptosis-Inducing Ligand , Tumor Cells, Cultured , Tumor Necrosis Factor-alpha , Metabolism , fas Receptor , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To observe the base of the interference in correlated biotic active matter obtained multi-drug resistance induced by chemotherapy for different alkaloid, and to supervise the use in clinic to restrain the multi-drug resistant of chemotherapy, and thereby to improve the curative effect.</p><p><b>METHOD</b>After bestowing subter-dosage unite chemotherapeutant to ascites S180 mouse to set up the mouse models of multi-drug resistance of S180 tumour cell, and giving the mouse matrine, terandrine, oxymatrine and berberine hydrooh loride for 4 weeks, the P170, LRP, TOPOII, Fas and apoposis were determined by flow cytometry.</p><p><b>RESULT</b>Matrine and terandrine could obviously reduce the express of P170, LRP and the activation of TOPOII, and increase the ratio of the express of Fas and the apoposis of drug resistant tumour cell. And at the same time it could obviously reduce the express of intercellular adhesion molecule(CD54).</p><p><b>CONCLUSION</b>Matrine and terandrine can interfere in MDR which results from chemotherapeutics by the adjustment of correlated biotic active matter, besides, the different degree of alkaloid effect with different configuration.</p>
Subject(s)
Animals , Female , Male , Mice , ATP Binding Cassette Transporter, Subfamily B, Member 1 , Metabolism , Alkaloids , Pharmacology , Apoptosis , Benzylisoquinolines , Pharmacology , Berberine Alkaloids , Pharmacology , DNA Topoisomerases, Type II , Metabolism , Drug Resistance, Multiple , Drug Resistance, Neoplasm , Plants, Medicinal , Chemistry , Quinolizines , Pharmacology , Random Allocation , Sarcoma 180 , Metabolism , Pathology , Tumor Cells, Cultured , Vault Ribonucleoprotein Particles , Metabolism , fas Receptor , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of tetrandrine on the P170 production expressed by multi-drug resistance gene, lung resistant protein (LRP), and topoisomeras II and elucidate the underlying molecular mechanism.</p><p><b>METHOD</b>Cellular model of multi-drug resistance was established in S180 tumor cell by means of the scheme of PFC chemotherapy at the dosage lower than that with curative effect. P170, LRP and TOPO II were measured by flow cytometry after the mouse model was treated with tetrandrine for 4 weeks.</p><p><b>RESULT</b>tetrandrine obviously reduced the enhancement of express of P170, LRP and the activity of TOPO II in the tumor cells with multi-drug resistance induced by chemotherapy.</p><p><b>CONCLUSION</b>Tetrandrine significantly inhibits the multi-drug resistance of tumor cells induced by chemotherapy via diminishing both the expression of multi-drug resistance gene and the activity of topoisomeras II.</p>