Histological Evaluation of Experimentally Induced Critical Size Defect Skin Wounds Using Exosomal Solution of Mesenchymal Stem Cells Derived Microvesicles

BACKGROUND AND OBJECTIVES: The present study investigated whether MSCs derived microvesicles (MVs) or (Exosomes) can exert therapeutic effects on an experimental model of cutaneous injury and explored the underlying involving mechanisms. METHODS AND RESULTS: Three bilateral full thickness circular wounds were created on the back of two groups of dogs using 2-cm dermal punch. The wounds were at least 2.5 cm apart. Saline was subcutaneously injected in 4 places around each wound area in group-I (control), whereas an equal volume of exosomal solution of MSCs derived MVs was similarly injected in group-II. The findings demonstrated that MSCs derived MVs had significantly promoted cutaneous wound healing, collagen synthesis, and vascularization at wound sites. The application of the exosomal solution had not only promoted the generation of newly formed vessels, but also have accelerated their development and maturation leading to a faster healing process. CONCLUSIONS: MSC-Exosomes appeared to be a superior candidate for treating cutaneous wounds than their originator cells, and may represent a promising opportunity to develop a novel cell-free therapy approach that might overcome the obstacles and risks associated with the use of native or engineered stem cells transplantation therapy.

Effect of "Flit"[TM] on the productive profiles and the histopathological changes of internal organs of adult albino rats

This formulated product as composed of the necessary amounts of pyrethroids and the required amount of the Kerosine caused severe, unacceptable pathological developmental changes. Body weight of newborns showed progressive affection of the newly born, of which Kerosine has marked influence. Mother behaviour was studied from quality of nest, post parturient aggressive interest of mother towards their youngsters were found to be significantly affected. Male sexual activity was revealed from weight of testis or accessory organs and semen quality. Kerosine alone induced significant differences. Estrous cycle was affected and regressive activity prolonged whatever the animal is exposed. Number of dead and resorbed foeti was significantly different in Kerosine and the whole compound. Teratogenic affection of the born foeti were clear even with Kerosine alone and absence of accessory elements [thymus gland]. Thus, this formulation is described as being severely hazardous and care should be taken even with the manipulation of Kerosine alone