ABSTRACT
AIM:To evaluate the therapeutic efficacy of intravitreal injections of ranibizumab for macular edema secondary to retinal vein occlusion(RVO)and the prognostic factors for this disorder.METHODS:A retrospective case study. There were 61 patients(61 eyes)with macular edema secondary to RVO who treated in our hospital from April 2020 to February 2021, including 30 cases(30 eyes)of branch retinal vein occlusion(BRVO)patients and 31 cases(31 eyes)of central retinal vein occlusion(CRVO)patients. All patients received 3 times of intravitreal injections of ranibizumab(0.5mg), and some eyes underwent retinal laser therapy. The patients were followed up for 3mo after treatment(the first intravitreal injection)to observe the visual acuity, intraocular pressure, central retinal thickness(CRT)and record the occurrence of ocular and systemic complications.RESULTS: The visual acuity of the included patients after treatment was significantly improved compared with that before treatment, and the CRT was significantly decreased compared with that before treatment(P<0.01), and after 3 times of intravitreal injections, the visual acuity of BRVO and CRVO patients with pre-treatment visual acuity≤1(LogMAR)was better than that of the patients with pre-treatment visual acuity>1(P<0.01), but there was no difference in CRT(all P >0.05). Among BRVO and CRVO patients, 12 and 8 eyes received retinal laser treatment during 3 times of intravitreal injections, respectively. The difference in visual acuity and CRT among the eyes treated with laser and those that were untreated was not significant(P>0.05). No ocular and systemic serious complications emerged during follow-up.CONCLUSIONS: Ranibizumab has high efficacy and safety in the treatment of macular edema secondary to RVO, while visual acuity at baseline may help predict the prognosis.
ABSTRACT
Objective: To investigate the effects of Cannabis Fructus oil on the regulation of gut microecology in D-galactose-induced aging mice. Methods: The Kunming mice were divided into control group, model group, positive control group (Jin Shuangqi group), Cannabis Fructus oil (12.0, 6.0, 3.0 mL/kg) group with six males and six females in each group. In the control group, normal saline was injected subcutaneously into the back of the neck every day. The other five groups were injected subcutaneously with 1% D-galactose aqueous solution, and the volume was 10 mL/kg. After 1 h, the control group was given normal saline by intragastric administration. The other groups were intragastrically administered with different doses of Cannabis Fructus oil for 42 d, and the dosage volume was 20 mL/kg. After the end of the administration, the change in body weight was analyzed; The proximal intestinal tissue of the ileocecal area and the feces in the cecum and colon were retained. Gram staining was used for the detection of Gram-positive bacilli (G+b), Gram-negative bacilli (G-b), Gram-positive cocci (G+c) and Gram-negative cocci (G-c); The ileal mucosa changes were observed by HE staining; The pH value of the colon feces was determined by pH meter; And the content of short-chain fatty acids (SCFA) in feces was determined by gas chromatography. Results: The results showed that Cannabis Fructus oil increased the ratio of bacillus, reduced the ratio of cocci and the cecal coefficient, decreased the pH value of the colon, significantly improved the colon pathological changes of the model animals with unbroken membrane skin, regular glands and rich cup cells and fluff rich, increased the content of SCFAs in the intestine of mice, and significantly increased (P < 0.01) the content of acetic acid, propionic acid, butyric acid, isobutyric acid, and significantly reduced (P < 0.01) the content of isovaleric acid. Conclusion: It could conclude that Cannabis Fructus oil can up-regulate the ratio of D-galactose-induced mice intestinal bacteria structure, improve the intestinal microecology, so as to provide theoretical support for clinical application and product development of Cannabis Fructus oil.
ABSTRACT
Objective To study the alteration of blood flow indexes of ophthalmic artery (OA),central retinal artery (CRA) and posterior ciliary artery (PCA) of the patients with cone-rod dystrophy (CRD).Methods The color doppler flow imaging was used in 50 CRD patients (100 eyes,CRD group) and 90 controls (180 eyes,control group) to measure the peak systolic velocity (PSV),end diastolic velocity (EDV),timeaveraged maximum velocity (TAMV),pulsatility index (PI) and resistance index (RI)in OA,CRA and PCA,respectively,and the data was obtained and analyzed statistically by one-sample t test to compare the alteration of these parameters between the CRD group and control group.Results The TAMV of OA was (14.29 ±3.88)cm · s-1 in CRD group and (12.44 ± 3.64) cm · s-1 in the control group,and the difference was statistically significant (P ＜0.05);meanwhile,the PI was 1.75 ±0.42 in CRD group and 2.02 ±0.71 in the control group,and the difference was statistically significant (P ＜0.05).Moreover,the PSV,EDV,TAMV and PI,RI of CRA was (4.60 ± 1.29) cm · s-1,(1.61 ±0.41)cm · s-1,(2.59 ±0.67)cm · s-1 and 1.11 ±0.31,0.63 ±0.10 in CRD group,respectively,and (10.82 ± 2.97) cm · s-1,(3.28 ± 1.11) cm · s-1,(5.50 ± 2.06)cm · s-1 and 1.48 ±0.49,0.71 ±0.08,in the control group,accordingly,and the differences were statistically significant (all P ＜ 0.05).Furthermore,the PSV,EDV,TAMV and PI,RI of PCA was (7.36 ± 2.18) cm · s-1,(2.28 ± 0.82) cm · s-1,(3.99 ± 1.22)cm · s-1 and 1.28 ± 0.37,0.68 ± 0.09 in CRD group,respectively,and (11.61 ± 3.41)cm · s-1,(3.34±1.25)cm · s-1,(5.83 ±1.91)cm · s-1 and 1.49 ±0.43,0.70 ±0.09 in the control group,accordingly,and the differences were statistically significant (all P ＜0.05).Conclusion Hemodynamic unusual alterations of OA,CRA and PCA in CRD patients suggest there is the correlation between the ocular blood supply and the pathological process of CRD,which remains to be observed further.
ABSTRACT
Retinitis pigmentosa is characterized by the progressive loss of the structure and function of photoreceptors and retinal pigment epithelium.Neurotrophic factors are more and more concerned.Brain-derived neurotrophic factors,ciliary neurotrophic factors and glial cell line-derived neurotrophic factors can affect photoreceptor activity through direct and indirect protection pathways.Neuronal-glial cell symbiosis system mediated by the indirect protection of photoreceptors is more important.Studies on glial cell-mediated neurotrophic factors for the treatment of related eye disease have made some progress,which will provide a new and effective methodsto delay the development of RP.
ABSTRACT
The taurine is one special kind of non-protein amino acid,possessing biochemical characteristics including regulation of NO activity,reduction of the content of free radical,anti-lipid peroxidation,regulation of calcium concentration,antagonism of glutamate toxicity,which can promote the differentiation and development of the retina.Given the characteristics above,taurine can be applied in the treatment of retinal diseases such as light-induced retinal injury,retinitis pigmentosa and diabetic retinopathy,and this paper will give a review about taurine for the treatment of retinopathy and its biochemical features.
ABSTRACT
<p><b>OBJECTIVE</b>To select the optimum extraction process of Zhanjin Ruji.</p><p><b>METHOD</b>To observe influence of extraction time upon the extraction rate of volatile oil, the orthogonal test was adopted to observe the extraction process by alcohol from the extraction rate and content of the total saponins in Radix Notoginseng.</p><p><b>RESULT</b>The three kinds of herbs including Radix Angelicae Sinensis, Resina Olibani and Myrrha were extracted with water for 3 hours, 95% of volatile oil can be distilled. The three kinds of herbs including Radix Notoginseng, Herba Lycopodii and Radix Gentianae Macrophyllac were extracted by alcohol. Four factors such as alcohol concentration(A), extraction times(B), extraction time(C), and solvent amount(D), had not significant effect on the content of total saponins in Radix Notoginseng in herbal extraction, but factor A and B had significant effect on the extraction rate. The optimum extraction process was as follows extracted with 5 times the amount of the solvent volum 60% alcohol for 3 times and with each time for 1 hour. Three times experiments showed that the extraction rate was 26.5% and the content of the total saponins in Radix Notoginseng was 17.28% mg.g-1.</p><p><b>CONCLUSION</b>The above experimental results can provide experimental basis for deciding the extraction process of Zhanjin Ruji.</p>