ABSTRACT
Demineralization of bone has been linked to the action of osteocytes via the process of osteocytic osteolysis. The aim of this study was to assess the influence of mechanical forces produced during orthodontic movements on the osteocytic lacunae. Orthodontic movements were achieved employing a device constructed [quot ]ad hoc[quot ] for rats that exerts a force of approximately 70 gr. The experimental animals and the corresponding controls were killed 48 and 96 hours after the onset of the experiment. Histologic sections oriented along the bucco-palatine axis were employed to measure the area of osteocytic lacunae to infer information on volume in keeping with standard stereological concepts. Regions alongside resorption areas of cortical bone and resting areas of palatine bone were evaluated. Osteocytic lacunae associated to erosive surfaces were rounded and rose markedly in area after the application of the orthodontic force (58.4 +/- 6 mm2). Elongated lacunae were present in relation to resting areas (24.8 +/- 2 mm2). The present study shows an association between the increase in size of osteocytic lacunae and the resorption fronts induced by the application of orthodontic forces. This finding would suggest that the osteocyte would participate in the resorption process of bone submitted to pressure.
ABSTRACT
Salivary glands are dependent on sexual hormones. The aim of the present work is to study the behavior of inflammatory response induced in animals that were castrated and injected with estrogens. Male adult wistar castrated rats (60-90 days) were used. A phlogogen pellet (zinc-oxide-turpentine essence) was placed between their sublingual and submaxillary glands and they were daily injected with 5 units of estrogen. The rats were killed after 8 and 12 days of treatment; submandibular pack was weighed, dissected and fixed in phormol Ph7 for its morphohistochemical study. Phlogogen pellet breaks out an acute inflammatory response that appears attenuated in castrated animals. Such character is enhanced when estrogens are used, disappearing ductal ectasis, becoming evident a granulation tissue of strange body in phagocytic activity and in contact with the pellet. As a consequence its follows that estrogen administration in castrated animals attenuates acute inflammatory response broken out by phlogogen pellet, determining characters with tendency to chronicity and giant cells differentiation of strange body.
ABSTRACT
En el presente trabajo se cuantifican mastocitos en relación a las modificaciones histológicas de las glándulas salivales de ratas sometidas a la castración y aplicación simultánea de DMBA. Se utilizaron machos jóvenes Wistar, en grupos de cinco animales cada uno. Se sacrificaron a los 12, 23 y 28 días de iniciada la experiencia. Se disecaron las glándulas y se fijan en formol a pH 7 para su estudio morfohistoquímico e histométrico. En la glándula sublingual (GSL) se incrementa el número de mastocitos en todos los plazos estudiados, superando significativamente la población celular de la glándula submaxilar (GSM) a los 12 días de castrados. Al comparar animales sin castrar y castrados se advierte que las diferencias más notables de la población mastocitaria se presentan en (GSL). Con DMBA y castración simultánea se observa que la glándula sublingual sin castrar) condiciona la respuesta tisular atípica de la glándula al efecto del cencerígeno y por ende a la población mastocitaria