Species and characteristics of protein adsorption on reused hemodialysis membranes / 生物医学工程学杂志

The aim of this study was to systemically analyze the proteins that adsorbed on the surface of hemodialysis membrane. The Fresenius F6 reused polysulfone dialyzers were selected as the research objects. The methodology we used encompassed the digestion of protein in rinsed solution and the separation of peptide mixture in virtue of RP-HPLC followed by ESI-MS/MS identification in orde to get their adsorption behavior, species and characteristics. The results illustrated that, after being rinsed by reverse osmosis (RO) water, 179 species of the protein adsorbed on the hemodialysis membrane, most of which were acidulous and middle or small weight protein molecules. The data from counting the numbers of peptides showed there were 5 species of high-abundant proteins (the contents being above 5% each), namely Ficolin-2 precursor, Complement C3 precursor (Fragment), Mannan-binding lectin serine protease 1 isoform 2 precursor, Complement-activating component of Ra-reactive factor precursor, and Mannan-binding lectin serine protease 1 isoform 3. These proteins are in close relationship with human immune system; moreover, they are of great significance to the clinical mechanism for cleaning reuse hemodialyzers and to the development of new hemodialysis materials.

Ingredients of membrane adhesion in reused dialyzer / 生物医学工程学杂志

Selecting reused polysulfone membrane (PSM) dialyzers as research objects, we mainly analyzed quantitatively the adhesion ingredients which obstructed the passage through the membrane, and we investigated the differences of residual contaminants on the surface of PSM in the cases of various reuse times. The results illustrated that after the completion of dialysis, the dialyzer was first cleaned by reverse osmosis (RO) water to remove the protein adsorpted. Then we used 2% sodium hypochlorite (NaClO) solution to soak it, and the glucose, cholesterol and triglyceride adsorpted were dissolved off. Meanwhile, the quantity of most of adsorption gradually increased with the increase of reuse times.

Development of Five-classification Technology in Hematology Analysis / 医疗卫生装备

The comprehensive analysis and comparison of the application and the technical character of a variety of hematology analyzer widely used in China's medical institutions is proposed on the basis of an introduction to the principle and technical features involved in the five-classification technology of the hematology analyzer, and the combined analysis on behalf of the five-classification technology is focused in above. Finally, the development trend of the hematology analyzer is summarized.