ABSTRACT
FMDV 2A peptide was introduced as a linker between GP5 and M protein of porcine reproduction and respiratory syndrome virus (PRRSV) to allow automatic self-cleavage the polyproteins. This strategy simultaneously displayed the neutralizing action of GP5 protein and cell-mediated immunity of M protein. We put them into the expression cassette of adenovirus vector. The results of RT-PCR, IFA and Western blotting showed that GP5 and M protein were not only expressed correctly, but also self-cleavaged and assemble heterodimers formation. To detect the advantages of rAd-GP5-2A-M, we also constructed some other recombinant adenoviruses (rAd-GP5, rAd-M and rAd-GP5-M) as control. After inoculated subcutaneously into BALB/c mice, the four recombinant adenoviruses can induce PRRSV-specific antibodies and cell-mediated immune response, but the level of humoral and cell-mediated immune response against PRRSV induced by rAd-GP5-2A-M is the strongest among the four recombinant adenoviruses. All of these suggested that it is possible to develop one multi-gene engineering vaccine utilizing FMDV 2A peptide, and also provided a novel strategy for developing other viral disease vaccine.
Subject(s)
Animals , Female , Mice , Adenoviridae , Genetics , Metabolism , Immunization , Mice, Inbred BALB C , Recombinant Fusion Proteins , Genetics , Allergy and Immunology , Metabolism , Swine , Vaccines, Synthetic , Allergy and Immunology , Viral Envelope Proteins , Genetics , Allergy and Immunology , Metabolism , Viral Matrix Proteins , Genetics , Allergy and Immunology , Metabolism , Viral Vaccines , Allergy and ImmunologyABSTRACT
Objective:To explore the cognitive styles and personality characteristics of patients with peptic ulcer.Methods:The personality characteristics,defense styles, and cognitive styles of 30 patients and 30 controls were evaluated.Results:(1)The patients group got higher scores on defense mechanism of sublimation,intercourse trend,consumptive trend and self-making than the control group.(2)Logistic analysis showed that some factors of DSQ were significantly different between the patient group and control group.(3)The interaction between personality and cognitive styles was also found in the analysis.Conclusion:Personality and cognitive styles together contributed to the onset and the course of peptic ulcers.