ABSTRACT
New l,3,8-trisubsttiuted purine-2,6-diones and 1,3,6-trisubstituted thiazolo[2,3-f] purine-2,4-diones were designed and synthesized as potential antitumor agents. The cytotoxic effects of the tested compounds were assessed against two human malignant-cell lines: T-cell leukemia derived SKW-3 and breast cancer -derived MDA-MB-231 using the methyl thiazolyl tetrazolium [MTT-dye] reduction assay, after 72 h exposure. The data -were fitted to sigmoidal concentration response curves and the corresponding 1C 50 values were calculated using commercially available software [GraphPad Prizm]. Compound AH-206 was the most potent cytotoxic agent among the newly synthesized compounds, with 1C 50 value of 17.3 micro M. Prominent activity was also encountered with compounds AH-201, AH-205, AH-208, AH-214 and AH-217, all having 1C[50] values below 100 micro M