Effect of ultrasound on the ultrastructure of rat skin and on the concentration of zinc and iron

This work deals with the use of ultrasound waves of frequency 20 KHz and average intensity 1.5 W/Cm[2], to study its effect on the cellular ultrastructure and the concentration of the zinc and iron on the rat skin. This study was conducted on 20 rats. They were divided into four groups: control, and three experimental groups exposed to ultrasound radiation for 15, 20 and 30 minutes respectively. Skin samples were taken to study thefr ultrastructure by a transmission electron microscope and their zinc and iron content by an atomic absorption spectrophotometry. There were evident on ultrastructural changes in rat skin, exposed to ultrasound at different intervals when compared with the normal tissue. As regard to the trace elements there was a significant decrease in concentration of Zn and increase in Fe concentration in comparison with the control group

Histopathological, ultra-structural and histochemical studies on the liver of schistosoma mansoni infected mice under the effect of triclabendazole

The present work investigated the effect of the fasciolicidal drug triclabendazole [TCBZ] on the liver of S. mansoni infected mice. The drug was given to normal as well as to S. mansoni infected mice. The work included histopathological, ultra-structural and histochemical studies. In the normal liver TCBZ induced the formation of scanty foci of inflammatory infiltrate. No changes were observed at the subcellular level or in the hepatocytes enzymes succinic dehydrogenase [SDH] and acid phosphatase [ACP]. The liver of S. mansoni infected mice revealed the classical histopathological picture of schistosomiasis. After TCBZ treatment, the granulomata involuted revealing fibrous transformation. The ultrastructural of hepatocytes of S. mansoni infected mice revealed distortion of the mitochondria, increased number of lysosomes and obliteration of Disse space. These electron microscopic [EM] changes were less obvious after TCBZ therapy denoting improvement of the hepatocellular insufficiency. Histochemically, an increase in ACP and a decrease in SDH activity were observed in S. mansoni infected liver. The activity of these enzymes returned to normal after treatment with TCBZ. It could be concluded that TCBZ has no direct toxic effect on the hepatocytes. In experimental schistosomiasis TCBZ improved the liver pathology and enzymatic activity of the hepatocytes

In situ hybrdization of epstein-barr virus encoded RNA [EBER] in aspiration samples in lymphoma

This study aimed at applying a non-isotopic in situ hybridization technique for EBV detection on fine needle aspiration [FNA] samples using the cell print instrument-blue supplied with 15 micro filter. A total of 35 selected FNA from lymph nodes, confirmed on biopsy basis, included 18 Hodgkin's disease, 12 cases of Non-Hodgkin's lymphoma and 5 cases of metastasis from nasopharyngeal carcinoma, used as control. All cases of Hodgkin's disease showed a condensed EBV positivity picked up by all mononucleated and binucleated Reed Sternberg cells, in all the subtypes; sparing other polymorphic reactive cellularity. In Non-Hodgkin's lymphoma cases, 10/12 cases were EBV negative except for insignificant occasional scattered weak positivity picked up by the resulting lymphocytes. The remaining 2 cases gave condensed EBV positivity in few scattered reticulum cells. Retrospective immunohistochemical staining by [CD [15, 20, 43]] for these 2 cases, proved to belong to Hodgkin's disease, lymphocytic predominance of B-cell type. All cases of metastatic nasopharyngeal carcinoma showed a condensed positivity in all the metastatic epithelial cells. The usefulness of applying ISH for EBV on aspirate samples on wider scale is recommended and the possible carcinogenic role of EBV in the evolution of various subtypes of Egyptian Hodgkin's disease is discussed

CD 30 [KI-1] antigen expression in anaplastic large cell tumours

The recently discovered monoclonal antibody Ki-1 [anti CD30], which recognizes an activated lymphocytic antigen, has been studied on 50 selected cases of large anaplastic tumors, easily mistaken morphologically with pleomorphic lymphoma or anaplastic carcinoma. All cases were subjected to a panel of 5 monoclonal antibodies, cytokeratin AE1/AE3, leukocytic common antigen [LCA], CD30 [Ki-1], L26 [B-cell marker] and CD43 [T-cell marker]. This study emphasizes the diagnostic validity of applying CD30 [Ki-1] marker for the confusing group of anaplastic large cell tumors of depict this unique entity of CD30 +ve anaplastic large cell lymphoma [ALCL]

Human fascioliasis: T cell subsets in liver before and after bithionol treatment

Liver biopsies from 5 patients with established fascioliasis, before and after bithionol treatment were studied by immunoalkaline phosphatase technique for relative distribution of T cells and their subpopulations. T cell and its subsets are defined for OKT3[+] [pan T], OKT4[+] [helper/inducer] and OKT8[+] [suppressor/cytotoxic] cells by using mouse monoclonal antibodies. Before bithionol treatment, lymphocytic infiltration in all hepatic lesions were predominantly of OKT3[+] [pan T] lymphocytes. The distribution of OKT8[+] cells was moderate to severe in comparison to the few OKT4[+] cells presentation. After bithionol a noticable regression of the OKT3[+] lymphocytic in all liver sections. The majority of the lymphocytic infiltration was of the OKT8[+] cells, in comparison to the absence of the OKT[4]+ ones. This may indicate that suppressor/cytotoxic lymphocytes may have a role in the immune regulation of the disease and the mode of action of bithionol is by the accentuation of this immunoregulartory effect

Human fascioliasis: ultrastructural study on the liver before and after bithionol treatment

The pathology of human fascioliasis was studied before and after bithionol treatment using light and transmission electron microscopy. Fine needle biopsies were taken from five patients, with established fascioliasis, before and after drug administration. By light microscope the pathology of human fascioliasis was similar to that reported in experimental fascioliasis. The ultrastructural picture revealed bile ductular hyperplasia, fibrosis of portal tracts, widening of the interhepatic spaces by many microvilli and dilated Disse space with collagen fibres. Bile ductular hyperplasia may be the initial factor to fibrinogenesis, which subsequenetly enhance the development of the microvilli on the surface of the hepatocytes. Both light and electron microscopic studies revealed regression of the picture of fascioliasis to normal after bithionol treatment with no sign of toxicity on the liver