ABSTRACT
Diabetes induces motor dysfunctions, Palmatine is an isoquinoline alkaloid, with anti-diabetic and antioxidant activities. This study was conducted to evaluate the effect of Palmatine on motor dysfunction in STZ-induced diabetic rats. In this experimental study, 32 male wistar rats were randomly allocated into control, Palmatine-treated non-diabetic, diabetic and Palmatine-treated diabetic groups. Diabetes was induced by STZ administration at the dose of 55 mg/kg/bw, intraperitoneally. Palmatine hydrochloride was administered subcutaneous at doses of 10 mg/kg/bw per day for a period of 6 weeks, one week after induction of diabetes. Blood glucose level was measured 1, 3, 5, 7 weeks after STZ injection. Locomotor activity tests including Y maze, grip-traction and inclined plane tests were performed to determining locomotor activity. In Y maze test, the number of arms entered significantly increased in Palmatine-treated diabetic group compared to diabetic group [P<0.05]. Grip traction and inclined plane tests significantly increased in Palmatine-treated diabetic group compared to diabetics animals [P<0.05]. Palmatine hydrochloride administration for 6 weeks improves motor dysfunctions in streptozotocin-induced diabetic rats
Subject(s)
Animals, Laboratory , Male , Gait Disorders, Neurologic/drug therapy , Diabetes Complications/drug therapy , Motor Activity , Hand Strength , Rats, Wistar , Diabetes Mellitus, ExperimentalABSTRACT
Recent studies have shown that diabetes induced cognitive dysfunction and impairs learning and memory. Palmatine is an isoquinoline alkaloid, and has multiple pharmacological effects, including anti-diabetic and antioxidant activity. This study was conducted, to evaluate the effect of Palmatine on learning and spatial memory impairment in STZ-induced diabetic rats. This experimental study was conducted on the male Wistar rats [n=32] with approximate weight of 240 +/- 40 grams. The rats were randomly allocated and were divided into 4 groups [n=8]: Control, Palmatine-treated non-diabetic, diabetic and Palmatine-treated diabetic groups. Diabetes was induced by STZ administration at the dose of 55 mg/kg through intraperitoneal route. Palmatine hydrochloride was administered subcutaneous at doses of 10 mg/kg/day 1 week after STZ injection for a period of 6 weeks. Blood samples were taken from the tail vein 1, 3, 5, 7 weeks after STZ injection to measure blood glucose levels. Behavioral tests including spatial recognition and objective recognition were performed at the end of study. Data were analyzed by using Prism-5, one way ANOVA and Tukey tests. In spatial recognition test, the number of entrance in new arm of the Ymaze, in the Palmatinetreated groups significantly increased in compare to diabetic group in both sixth and seventh weeks [P<0.05]. Number of rearing in new arm significantly increased in sixth and seventh weeks, compare to the diabetic group [P<0.05]. The number of recognition novel objects in the Palmatine-treated diabetic group significantly increased in compare to diabetic group [P<0.05]. Palmatine hydrochloride administration for 6 weeks improves cognitive dysfunction in streptozotocin-induced diabetic rats
ABSTRACT
Background: Increasing evidence has shown that diabetes induces cognitive dysfunction and impairs learning and memory. Berberine, which is an isoquinoline alkaloid with multiple pharmacological effects including anti-diabetic and antioxidant activity, could improve learning and spatial memory impairment in STZ-induced diabetes in rats
Material and methods: Male Wistar rats [n = 40] were randomly allocated and similarly grouped into control, berberine-treated non-diabetic [100 mg/kg], diabetic, and berberine-treated diabetic [50, 100 mg/kg] groups. Diabetes was induced by STZ administration at the dose of 55 mg/kg through i.p. route. Berberine hydrochloride was administered p.o. at doses of 50 and 100 mg/kg/day 1 week after STZ injection for a period of 6 weeks. Blood samples were taken from the tail vein 1, 3, 5, and 7 weeks after STZ injection to measure blood glucose levels. Behavioral tests, including spatial recognition and objective recognition, were performed at the end of the study as described below
Results: Berberine-treated diabetic groups [50, 100 mg/kg/day] showed dose-dependent significant differences with diabetic group in spatial recognition and objective recognition tests, including the number of entries into the new arms and also the number of rearings into the new arms of Y maze. Moreover, compared with the diabetic group, objective recognition test score increased in berberine-treated diabetic group [50, 100 mg/kg], but not significantly
Conclusion: Berberine hydrochloride administration for 6 weeks improves cognitive dysfunction in streptozotocin-induced diabetic rats
ABSTRACT
In traditional medicine "Crataegus melanocarpa" is one of the medicinal plants that has many therapeutic effects. One of them is its effect on nerveous system. Evaluation of Intracerebroventricular [ICV] injection of alcoholic extract of "crataegus melanocarpa" leaves on fear behavior in presence pentylenetetrazole [PTZ] in adult male rat. Animals were divided into 3 groups: experimental, saline and, pentylenetetrazole [PTZ] 20mg/kg as positive control [I.P]. In the experimental group, the animals received 4000 microg/Rat alcoholic extract of "Crataegus melanocarpa" leaves [ICV] and PTZ [I.P]. Then plus maze was used for evaluation of the fear. Open arm entries percentage [%OAE] and open arm time percentage [%OAT] were evaluated. The results show that intracerebroventricular [ICV] injection of alcoholic extract of "Crataegus melanocarpa" leaves increased%OAE and%OAT significantly [p<0.05]. We concluded that alcoholic extract of "Crataegus melanocarpa" is effective on fear reduction in presence pentylenetetrazole [PTZ] in adult male rat