ABSTRACT
The purpose of this experimental study was to evaluate the effect of chicken amniotic fluid [AF] on a cross section of rat sciatic nerves. Thirty adult male Sprague-Dawley rats weighing 275 to 300 g, were randomized into three groups treated with [1] amniotic fluid or AF [n=10], [2] normal saline or NS [n=10], and [3] sham surgery [n=10]. The AF was aspirated from the amniotic cavity of incubating chick embryos at day 14. The sciatic nerve was exposed and sharply transected. Immediate epineurial repair was then performed. AF treated animals were given 2 ml/kg of the chick embryo AF subcutaneously, once daily, five times a week for up to 2 weeks. All animals were evaluated by sciatic functional index [SFI], electrophysiology, histology, and immunohistochemistry at days 28 and 56 after surgery. The SFI difference between AF and NS groups at days 21 and 28 after operation was statistically significant [P<0.05]. The number of myelinated fibers in the AF group was significantly greater than that of the NS group at day 28 [P<0.05]. At days 28 and 56 after operation, the nerve conduction velocity [NCV] mean of the AF group was faster than that of the NS group, but the difference was not statistically significant [P>0.05]. The results of this study demonstrate that chick AF can enhance peripheral nerve regeneration
ABSTRACT
Gonadotropin-releasing hormone [GnRH] has neuromodulatory roles in central and peripheral nervous systems. The purpose of this study is to evaluate the effect of GnRH analog [buserelin] on peripheral nerve regeneration. Forty adult male rats were divided into buserelin-treated, normal saline, sham surgery, and castrated + buserelin groups. The left sciatic nerve was crushed by a fine forceps and all animals were evaluated by sciatic functional index [SFI], electrophysiology, histology and immunohistochemistry testing. On post operation days 21 and 28, the difference between buserelin and normal saline groups was statistically significant [P<0.05], but no significant difference was found between the buserelin and castrated + buserelin groups[P>0.05]. At the 28th day after operation, the diameters [microm] of the regenerated myelinated fibers of the buserelin group were significantly greater than those of the normal saline group [P<0.05]. Although nerve conduction velocity [NCV] of the buserelin group was faster than the normal saline group, the difference was not statistically significant. The present study suggests that buserelin treatment might accelerate peripheral nerve regeneration
ABSTRACT
The epididymis is a tubule that processes the maturation, storage, and transfer of sperm. Growth and maintenance of epididymal structures depend on testosterone release, which is directly controlled by pituitary gonadotropins. Furthermore, gonadotropins are controlled by hypothalamic releasing hormones. Using gonadotropin releasing hormone [GnRH] analogs can stop the pituitary-gonadal axis. This study aimed at determining the effect of a GnRH agonist [buserelin] on prepubertal rat epididymal tissue. In this experimental, 20 rats on the 25[th] day after birth, provided by the Department animal house, were divided into 4 groups. The first and second study groups received 0.1 mg/kg GnRH agonist for 5 days and were dissected on the 30th and 35[th] day after birth, respectively. Animals in the control group received physiologic serum 0.1 mg/ml for 5 days and were dissected like the study groups. Measurements were performed using a calibrated microscope. The findings reveal a significant increase in epithelial height and lumen diameter in the first and second study groups as compared with the first control group [p