ABSTRACT
Despite the establishment of extensive and successful control programmes in some countries or regions, Echinococcus granulosus still has a very wide geographical distribution. Mature E. granulosus [n = 120] obtained from Parasitology Department, School of Veterinary Medicine, Ferdowsi University of Mashhad [Iran]. Soluble protein of whole body of parasite was prepared by freeze-thawing in liquid nitrogen and at 42°C for three times. The sample was homogenized in a blender, sonicated at 110 V, 170 W for 3 times for 15 s each and then centrifuged at 10,000 chi g for 15 min. Final yield was kept in -20