Use of milk progesterone radioimmuno assay and enzyme-immunoassay in the diagnosis of pregnancy in buffaloes

In view of comparing between milk progesterone radioimmunoassay and enzyme immunoassay for diagnosis of pregnancy in buffalos, 140 whole milk samples were collected from 20 cows on days 18th, 19th, 20th, 21st, 22nd, 23rd and 24th after mating. The milk samples were analyzed for progesterone with RIA and EIA. Applicability, reliability and the values of progesterone measured by both methods were compared. This study indicated that days 20th, 21st or 22nd is the most reliable for performing this analysis for pregnancy diagnosis in buffalos