ABSTRACT
OBJECTIVE: To determine frequency of Vitamin B12 deficiency in subjects with anemia and elevated mean corpuscular volume
STUDY DESIGN: Cross sectional study
PLACE AND DURATION: Department of Medicine, Liaquat University and Isra University Hospital Hyderabad from January 2011 to August 2013
METHODOLOGY: A sample of 113 subjects was selected according to inclusion and exclusion criteria. Serum vitamin B12 deficiency was defined as per standard criteria. Blood samples were analyzed on Sysmex KX 21 hematology analyzer. Continous variables were analyzed by ANOVA and Tukey-Cramer test while categorical variables by Chi-square test
The association between variables was analyzed by Pearson`s correlation. A p-value of
RESULTS: Of 113 subjects, 37 [32.7%] were male and 76 [67.2%] female, mean+/-SD age was 34.48+/-6.71 years and 89.3% [n=101] were anemic. Mean hemoglobin, hematocrit and RBC counts were found as 11.3 g/dl, 33.1% and 3.0 million/µL respectively. Of total 113 subjects, vitamin B[12] deficiency was noted in 65 [57.5%]; further subdivided as borderline and definitive deficiency in 19.4% and 37.9% respectively. Mean corpuscular volume as high as 139 fl and vitamin B[12] levels as low as <30pg/ml were found. Pancytopenia was noted in 11 [9.7%] with severe vitamin B[12] deficiency [<100pg/ml]. Significant negative correlation was found between vitamin B[12] and mean corpuscular volume [r=-0.79, p=0.0001]. Peripheral blood film revealed anisocytosis, poikilocytosis, megaloblasts and hypersegmented neutrophils
CONCLUSION: Vitamin B12 deficiency is prevailing and is a major contributing factor of megaloblastic anemia
ABSTRACT
Objective: To assess the early detection of thrombocytopenia in women presenting with varying degree of pregnancy induced hypertension [PIH]. Study Design: A case control study. Place of Study: Hematology laboratory Isra University Hospital Hyderabad. Duration of Study: From July 2009 to December 2010
Materials and Methods: Total 130 pregnant women were included in this study. The subjects were divided into three groups as Group 1 with pre-eclampsia, Group 2 with eclampsia and Group 3 with normotensive pregnant women as control group. The Group 1 was further divided into two sub groups such as Subgroup 1a with mild preeclampsia and Sub-group 2b with severe pre-eclampsia
Results: Anticoagulated whole blood samples [5cc] from all subjects were analyzed for the detection of thrombocytopenia for the possible involvement of pregnancy induced hypertension. It was noted that out of total subjects, 33[25.39%] had mild pre-eclampsia, 17[13.07%] had severe preeclampsia, 15[11.54%] had eclampsia and 65 [50.0%] were normotensive pregnant women. Based on the comparative findings, the results showed significant differences between group 3 and group 1a [p-value 0.001], group 3 and group 1b [p-value 0.001], group 2 and group 3 also showed same results [p-value 0.001] but the subjects of group 1a and 1b when compared, showed non-significant findings [p value 0.955]
Conclusion: The results suggested that early detection of platelet count provide significant role for the assessment of severity of disease in women with pregnancy induced hypertension when compared with normotensive pregnant women