Diphtheria anatoxin industrial purification by gel filtration

Diphtheria toxoid is prepared from the toxin produced by fermentative process, of the Park-Williams N§8 strain of Corynebacterium diphteriae grown on F-20 medium. The toxin separeted from the organisms is detoxified and purified. Diphtheria toxoid is the immunizing antigen protecting against the adverse effects of infection by diphtheria organisms and rarely is used alone. It is used in associated vaccines with tetanus toxoid and pertussis vacine. This paper describes the separation of diphtheria anatoxin proteins on Sephadex G-50, Sephacryl S-100 HR and Superdex 75 prep grade

Estabelecimento da concentracao antimicrobiana eficaz de tiomersal em toxoide tetanico / Determination of effective antimicrobial concentration of thimerosal in tetanus toxoid

Amostras de taxoide tetanico, contendo quatro concentracoes de tiomersal (0,005; 0,010; 0,015 e 0,020%) dentro dos limites recomendados oficialmente,foram submetidos ao teste de eficacia antimicrobiana do conservante. Com relacao as exigencias da Farmacopeia Americana XXII, apenas Staphylococcus aureus se mostrou resistente as quatro concentracoes, enquanto que para Pseudomonas aeruginosa, Candida albicans e Aspergillus niger o sistema conservador atendeu as exigencias oficiais, mesmo na menor concentracao. O teor de tiomersal em toxoide tetanico podera ser de 0,005%