ABSTRACT
Objective: We aimed to investigate the oxidative stress status in children with beta-thalassemia major [beta-TM] by measuring native thiol [SH], disulfide [SS] and total thiol [SH + SS] plasma levels
Methods: This study was carried out from November 2017 to March 2018 at the Pediatric Hematology Clinic of the Harran University Medical Faculty Hospital. Blood specimens were collected from 100 participants, including 50 beta-TM patients and 50 controls, and SH, SS and SH+SS levels were detected through a newly developed method
Results: SH, SS, SH+SS levels and SS/SH ratio were markedly higher in beta-TM patients than in controls. In beta-TM group, SH and SH+SS levels were positively correlated with age, albumin and total bilirubin. Serum ferritin level was positively correlated with SH, SH+SS, aspartate transaminase and alanine transaminase
Conclusions: We found that the SS/SH ratio was high in patients with beta-TM, which shows increased oxidative stress. This ratio may be considered as a tool for the determination of oxidative status in such patients due to easily calculate, suitable for routine use and economical