ABSTRACT
The present study thus aimed at the development and physicochemical characterization of solid lipid nanoparticles loaded with crude extract of Piper corcovadensis roots (SLN - CEPc) and chitosan - coated solid lipid nanoparticles loaded with crude extract of P. corcovadensis roots (C - SLN - CEPc), as well as the determination of its antimycobacterial activity against Mycobacterium tuberculosis H37Rv, its cytotoxicity against the Vero cell line and evaluation in the hemolysis assay. Both formulat ions containing the encapsulated extract showed high encapsulation efficiency, formed by a monodispersed system with small and spherical particles, and there was no aggregation of particles. In the biological assays, SLN - CEPc and C - SLN - CEPc showed promisin g anti - M. tuberculosis activity with a minimum inhibitory concentration (MIC) of 12.5 µg/mL, whereas the cytotoxic concentrations obtained at 50% (CC 50 ) in Vero cells were 60.0 and 70.0 µg/mL, respectively. Therefore, nanoencapsulation showed satisfactory results, justifying its usage in the development of new products.
El presente estudio apuntó al desarrollo y caracterización fisicoquímica de na nopartículas lípidas en estado sólido, cargadas con extracto crudo de raíz de Piper c orcovadensis (SLN - CEPc) y nanopartículas lípidas en estado sólido cubiertas con quitosano cargadas co n extracto crudo de raíz de P. corcovadensis (C - SLN - CEPc), así como la determinación de su actividad antimico bacterial contra Mycobacterium tuberculosis H37Rv, su citotoxicidad contra la línea celular Vero y su evaluación en ensayo de hemólisis. Ambas formulaciones que contenían el extracto encapsulado mostraron alta eficien cia de encapsulación, formado por un sistema monodispersado con pequeñas partículas esféricas, y no hubo agregación de partículas. En los ensayos biológicos, SLN - CEPc y C - SLN - CEPc mostraron un a prometedora actividad anti - M. tuberculosis con una mínima conc entración inhibitoria (MIC) de 12,5 µg/mL, mientras que las concentraciones citotóxicas obtenidas al 50% (CC 50 ) en células Vero estuvo en 60,0 y 70,0 µg/mL, respectivamente. Por lo tanto, la nanoencapsulación mostró resultados satisfactorios, justificando su uso en el desarrollo de nuevos productos.
Subject(s)
Plant Extracts/administration & dosage , Drug Delivery Systems , Piper/chemistry , Anti-Bacterial Agents/administration & dosage , Mycobacterium tuberculosis/drug effects , Temperature , Drug Carriers , Chromatography, High Pressure Liquid , Plant Roots , Chitosan , Nanoparticles , LipidsABSTRACT
The main purpose of this work was to produce a pellet to minimize undesirable effects in fixed beds, such as colmatation, through clay fixation on the surface of polymeric particles. Preliminary experiments were carried out by heating the clay, namely Tonsil Terrana 580FF, in order to observe variations on its capacity to adsorb anthocyanins derived from red cabbage. Clays was fixed on five samples of high density polyethylene (HDPE) at 180°C -210°C for two hours. The morphological analyses of the resulting particles were accomplished through Scanning Electron Microscopy (SEM). The experiments demonstrated an increase on clay's adsorptive capacity of 15.65 percent at 120°C for 30 hours and 16.80 percent at 170°C for two hours. The SEM analysis showed that the clay particles adhered on the external surface of the pellets .These results show that it is possible to obtain polymeric pellets using HDPE coated with clay.