ABSTRACT
Peroxisome Proliferator-Activated Receptor gamma (PPARγ) is a nuclear receptor family transcription factor that is expressed in several types of cancers. Antiproliferative and proapoptotic actions of PPARγ agonists suggesting that, it could be a promising therapeutic target for the treatment of variety of cancers. Therefore, the main aim of this study is attempt to identify the novel PPARγ agonist from presence of bioactive compounds in ethanolic extract of Cayratia trifolia using GC-MS analysis and Computational molecular simulation studies. The GC-MS analysis revealed that the ethanolic extract of Cayratia trifolia (L.) (whole plant) consist of 20 bioactive compounds which embrace many biological activities against variety of diseases. Molecular docking studies (Glide 5.5 from Schrödinger suite) exposed that, out of 20 bioactive compounds, Cyclopentadecane, 9- Borabicyclo [3.3.1]nonane, 9-(2-propen-1-yloxy)-.1, 4,8,12,16-Tetramethylheptadecan-4- olide, Oxirane and Vitamin E shows the better glide score. ADME properties (QikProp 2.3 from Schrödinger suite) of these bioactive compounds were under the acceptable range. Based on the result it can be concluded that, these bioactive compounds may act as a good agonist for PPARγ. In future it may focus on current discoveries in PPARγ activation and possible anticancer therapeutic option.
ABSTRACT
The aim of the study was to investigate the invivo antioxidant activity of ethanolic extract Premna corymbosa (Rottl.) root against streptozotocin induced oxidative stress in different organs (liver, kidney, brain, heart and pancreas) of rats. Ethanolic extract of P.corymbosa (Rottl.) root was administered orally (200 mg/kg body weight) and the effect of extract on enzymatic antioxidants like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and polyphenol oxidase (PPO), non enzymatic like vitamin C, vitamin E and glutathione. Lipid peroxidation like basal, ascorbate and peroxide induced lipid peroxidation were also estimated. Glibenclamide was used as standard reference drug. A significant increase in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-Stransferase and reduced glutathione were observed in different organs of diabetic rats on treatment with 200 mg/kg body weight of P.corymbosa (Rottl.) root extract and glibenclamide for 30 days treatment. Both the treated groups showed significant decrease in lipid peroxidation, suggesting its role in protection against lipid peroxidation induced membrane damage. P.corymbosa (Rottl.) possesses antioxidant potential which may be used for therapeutic purposes mainly in the prevention of oxidative damage that occur during diabetes.
ABSTRACT
The study was formulated with the objective to assess phytochemical constituents, antibacterial activity and anticancer activity of Alpinia purpurata.