ABSTRACT
Pippalimula (root of Piper longum Linn.) is the spice of Indian cuisine having immense therapeutic properties. The study was conducted to evaluate Physicochemical, phytochemical analysis and HPTLC analysis using specific solvent system for Pippalimula. Material and Methods: Fine powder of Grade I Pippalimula (root of Piper longum Linn.) and alchoholic extract were obtained and subjected to phytochemical analysis and chromatographic study. Discussion: Physicochemical analysis of the root was carried out. HPTLC analysis of the methanolic extract of the root powder was performed with Toluene: Ethyl acitate 9:1.(v/v) Conclusion: HPTLC analysis of root of Piper longum Linn.can provide standard analysis with selected solvent system and can be used as a reference for the authentication and quality control of the drug. The study will provide referential information for the correct identification of the crude drugs.