ABSTRACT
Antimutagenic potential of a fraction isolated from Terminalia arjuna has been evaluated in TA98 and TA100 strains of Salmonella typhimurium against direct and indirect-acting mutagens. The fraction was quite effective against S9-dependent 2AF while it showed moderate effect against NPD. The fraction was analyzed to be ellagic acid.
Subject(s)
Antimutagenic Agents/isolation & purification , Ellagic Acid/isolation & purification , Mutagenicity Tests , Plants, Medicinal/chemistry , Salmonella typhimurium/geneticsABSTRACT
Antimutagenic effect of 2 polyphenolic fractions isolated from T. bellerica in TA98 and TA100 strains of S. typhimurium against 2AF, NPD and 4NQNO has been characterized. Both the fractions were significantly effective against S9-dependent 2AF; less effective against NPD and almost not effective against 4NQNO in TA100 strain. Using 13C-NMR spectral analysis, the TB-3 fraction, which was significantly more effective against 2AF compared to TB-4, was found to be a mixture of 3 tannins while TB-4 was non-tannin fraction. Interaction of polyphenols with S9 proteins may be the probable cause of inhibitory effect of these polyphenols, though the possibility of other mechanisms cannot be ruled out.
Subject(s)
Antimutagenic Agents/isolation & purification , Flavonoids , Fruit/chemistry , Phenols/isolation & purification , Polymers/isolation & purification , Salmonella typhimurium/drug effects , Species SpecificityABSTRACT
Antimutagenicity of water and chloroform extracts of dried myroblan Terminalia chebula was determined against two direct acting mutagens, sodium azide and 4-nitro-o-phenylenediamine (NPD) in strains TA100 and TA1535, and TA97a and TA98 of Salmonella typhimurium respectively and S9-dependent mutagen 2-aminofluorene (2-AF) in TA97a, TA98 and TA100 strains. Water extract reduced NPD as well as 2-AF induced his+ revertants significantly but did not have any perceptible effect against sodium azide included his+ revertants in TA100 and TA1535 strains of S. typhimurium. The pre-incubation studies, where the extract was incubated at 37 degrees C for 30 min with the said mutagen prior to plating, enhanced the inhibitory effect. Autoclaving the water extract reduced the inhibitory effect but the reduction in the effect was not significant. No inhibitory effect was observed in any of the strains and against any of the test mutagens with chloroform extract.
Subject(s)
Chloroform , DNA/drug effects , Dose-Response Relationship, Drug , Mutagenicity Tests , Plant Extracts/pharmacology , Plants, MedicinalABSTRACT
Mutagenicity of metasystox and rogor could not be detected on the basis of tests employing Ames Salmonella/microsome assay even in the presence of wheat seedling or rat liver microsomal activation systems.
Subject(s)
Animals , Dimethoate/toxicity , Mutagenicity Tests , Mutation , Organothiophosphorus Compounds/toxicity , Pesticides/toxicity , Rats , Salmonella typhimurium/drug effectsABSTRACT
Water, acetone and chloroform extracts of E. officinalis fruit reduced sodium azide and NPD induced his+ revertants significantly in TA100 and TA97 a strains respectively of S. typhimurium. The chloroform extract was less active as compared to water and acetone extracts. Autoclaving of water extract for 15 min did not reduce its activity. The enhanced inhibitory activity of the extracts on pre-incubation suggests the possibility of desmutagens in the extracts. Besides ascorbic acid, a constituent of the extract, the role of other antimutagenic factors in the extract cannot be ruled out.