ABSTRACT
Virus isolation tests were performed during the first, second, and third trimesters of pregnancy on a group of 163 students who received prenatal care at the University of Chile's Student Medical and Dental Service to determine the possible existence of genital herpes or cytomegalovirus infections. Vaginal secretion samples tested for herpes simplex virus (HSV) and urine samples tested for cytomegalovirus (CMV) were inoculated onto cell cultures and examined by immunofluorescent procedures using monoclonal antibodies. HSV was detected in five subjects, only one of whom had herpetic lesions, and was identified as type 2 in all cases. Cytomegalovirus was detected in seven subjects
Subject(s)
Cytomegalovirus/isolation & purification , Students , Simplexvirus/isolation & purification , ChileABSTRACT
En un grupo de 163 estudiantes embarazadas que acuedieron al Servicio Médico y Dental de Alumnos de la Universidad de Chile (Santiago) para control ginecobstetrico en el 1er, 2o y 3er trimestre del embarazo se investigo mediante pruebas de aislamiento virico la posible existencia de infeccion genital herpetica (aislamiento en secrecion vaginal) o infeccion por citomegalovirus (aislamiento en orina). Las muestras fueron inoculadas en cultivos celulares y se investigaron luego mediante inmunofluorescencia con anticuerpos monoclonales. Se detecto virus del herpes simple (VHS) en la secrecion vaginal genital de 5 embarazadas, de las que solo una tenia lesiones herpeticas; en todos los casos se aislo VHS tipo 2. Los citomegalovirus se detectaron en 7 de las embarazadas
Subject(s)
Cytomegalovirus/isolation & purification , Students , Simplexvirus/isolation & purification , ChileABSTRACT
This article reports on the prevalence of genital herpes infection in a sample of 635 female university students who were seen at the Gynecology Center of the University of Chile Student Medical Service. Viral isolation of genital secretions yielded 18 students (2.8 percent) with positive results, of whom 28 percent were asymptomatic shedders. Of the positive cases, 61 percent were clinically classified as recurrent and 39 percent as first episodes of genital herpes. Fluorescent monoclonal antibody typing of the isolated strains revealed that 72 percent were herpes simplex type 2 and 28 percent were type 1. The results confirm the importance of type 2 virus in genital herpes infection. They also point to asymptomatic shedders as a potential source of infection, and show the importance of a history of genital herpes in the identification of this population