ABSTRACT
Objective To investigate the application and correlation of enterovirus 71(EV71)and Coxsackie virus A16(CA16)antibody screening in the hand,foot and mouth disease(HFMD).Methods The method of enzyme linked immunosorbent assay was used to test the serum antibody of EV71-IgM and CA16-IgM in 281 patients(142 male and 139 female)with HFMD.Results The positive rate of EV71-IgM antibody was 51.2％(144/281),the positive rate of CA16-IgM antibody was 32.0％(90/281).Seventeen patients(6.0％,17/281)had dual positive of EV71-IgM and CA 16-IgM antibody.The total detection rate was 77.2％(217/281).The positive rate of EV71-IgM and CA16-IgM antibody had significant difference(x2 =21.35,P ＜ 0.01).The positive rate of EV71-IgM and CA16-IgM antibody in male had significant difference compared with female[95.1％(135/142)vs.71.2％(99/139),P＜ 0.01].Conclusions At the beginning of the illness should use the joint detection of EV71-IgM and CA16-IgM antibody diagnose HFMD.It has significance in diagnosing and monitoring.