ABSTRACT
Objective To compare the expression of myocardial connexin 43(Cx43)mRNA and its protein during early and late cardioprotection stages of exercise preconditioning.Methods Thirty-two Sprague-Dawley rats were randomly divided into a control group,an exhaustive exercise(EE)group,an early exercise preconditioning + exhaustive exercise(EEP+EE)group and a late exercise preconditioning + exhaustive exercise (LEP+EE) group,each of 8.All groups were given intervention as their group name indicated.Then in situ hybridization and real-time fluorescent quantitative PCR methods were used to detect the changes of myocardial Cx43mRNA and immunohistochemical method and Western blotting were used to detect the changes of Cx43 protein.Results Compared with EE group,there was significant increase in Cx43 mRNA and its protein expression in group EEP+EE and LEP+EE.Compared with EEP+EE group,no significant changes was found in situ hybridization and Cx43 Immunoreactivity in LEP+EE group,neither did significant differences in the expression of Cx43 mRNA and its protein.Conclusion EEP and LEP can significantly promote the expression of myocardial Cx43 mRNA and its protein respectively.However there is no significant changes of myocardial Cx43 mRNA and protein expression between the 2 time phases.It demonstrates that the expression of Cx43 in the early and late stage of myocardial protective effect was consistent with the changes of the early and late phase of the protective effect of EP.
ABSTRACT
Objective: To explore how social support in students of normal college related to their interpersonal attribution and philosophies of human nature, and whether social support varied with different subgroups. Method: Social Support Rating Scale, the Multidimentional-Multiattributional Causality Scale and Revised Philosophies of Human Nature Scale were used in 414 students of a normal university. Result: The social support of students differed significantly among different departments, students of science had lower social support than students of art or sport had. There was no significant difference of social support between sexes, different grades, of urban or rural area. Social support was significantly correlated with "effort" attribution of interpersonal relationship, but not with "ability, background or luck". Social support was positively related to trustworthiness, while negatively to cynicism. Conclusion: This result fits for the common sense of Chinese and people elsewhere.