ABSTRACT
Objective To study the antitumor effects of Scutellaria Radix extracts on H22 transplanted tumor in mice and its possible mechanism.Methods The H22 transplanted mouse models were established by inoculating H22 to mice. 60 mice were randomly divided into model group, 5-Fu group, low-, medium- and high-dose Scutellaria Radix extracts groups, with 12 normal mice as control group. All administration groups received gavage with relevant medicine. And then the body weight change, tumor growth inhibitory rate, and spleen and thymus indexes were calculated;the T-AOC and activities of SOD, and MDA content in serum were detected by spectrophotometer;DNA damage of peripheral blood lymphocytes was observed by single cell gel electrophoresis.Results Medium- and high-dose Scutellaria Radix extracts can significantly inhibit the growth of transplanted tumor. Compared with 5-Fu group, medium- and high-dose Scutellaria Radix extracts groups notably increased the body weight, spleen and thymus indexes of mice, promoted T-AOC and activities of SOD, and decreased MDA content in serum, as well as notably reduced DNA damage of peripheral blood lymphocytes.Conclusion Scutellaria Radix extracts have obvious antitumor effects on H22 transplanted tumor in mice and the possible mechanisms may be due to lightening the damage of normal tissue and cell from tumor, enhancing immunologic function and improving antioxidant capability of H22 bearing mice.
ABSTRACT
Objective To explore liver protective effects of the stems and leaves and roots of Scutellariae Radix from different areas of Gansu Province;To discuss differences in liver protection of the stems and leaves and roots of Scutellariae Radix. Methods All Kunming mice were randomly divided into 11 groups:normal group, model group, positive control group, Scutellariae Radix root groups and Scutellariae Radix stem-leaf groups of Longxi, Minxian, Weiyuan, Zhangxian. The mice received gavage with relevant medicine once a day for 7 days. 2 hours after the last medication, the mice were given peritoneal injection with 0.1%CCl4 and olive oil to establish the models with liver injury. Hepatic index, the activity of serum ALT and AST, the levels of T-AOC in the liver tissue were calculated in 18 hours after establishing the models. Results Compared with the model group, hepatic index, the activity of serum ALT and AST in the mice of Scutellariae Radix root groups from difference producing areas decreased significantly (P<0.05);the levels of T-AOC in the liver tissue was raised notably (P<0.05). Compared with stem-leaf groups from the same producing area, hepatic index, the activity of ALT and AST in the mice of Scutellariae Radix root groups were remarkably lower than these of Scutellariae Radix stem-leaf groups (P<0.05);the levels of T-AOC in the liver tissue was evidently higher than that of Scutellariae Radix stem-leaf group (P<0.05). Conclusion Scutellariae Radix root water decoction can prevent and protect CCl4-induced acute liver injury to a certain degree, while Scutellariae Radix stem-leaf water decoction shows no significant protective effect on acute liver injury.