ABSTRACT
Objective To investigate the effect of overexpression of miRNA-4465 on invasion and metastasis of breast cancer MDA-MB-231 cells and its mechanism.Methods MiRNA-4465 minics was transfected into the MDA-MB-231 cells,and real-time PCR was used to detect the expression of miRNA-4465.Transwell assay was used to investigate the migration and invasion capability of cells after being transfected.Bioinformatics analysis were performed to predict the potential targets of miRNA-4465,and finally,luciferase reporter plasmids assay and west-ern blot was used to confirm the potential target of miRNA-4465.Results Comparing with negative control group, the expression of miRNA-4465 in miRNA-4465 minics group was increased significantly.Transwell migration assay showed that migration capability of cells in miRNA-4465 minics group was decreased(P =0.001);transwell inva-sion assay showed that invasion capability of cells in miRNA-4465 minics group was decreased(P =0.010).Lucif-erase reporter plasmids assay showed that comparing with the negative control +psiCHECK2-EZH2 3’UTR(wild type),the fluorescence activity of miRNA-4465 minics +psiCHECK2-EZH2 3’UTR(wild type)was decreased by 66%(P =0.001).Moreover,comparing with the negative control group,the expression of EZH2 protein was de-creased after transfecting miRNA-4465 minics into MDA-MB-231 cells.Conclusion MiRNA-4465 can suppress the invasion and metastasis of breast cancer MDA-MB-231 cells,which may be related to the regulation of EZH2.
ABSTRACT
Objectives:Survey the prevalence rate and correlation risk factors of first diagnosed type 2 diabetes(T2DM)with metabolism syndrome(MS).Methods:We analize the prevalence rate and correlation risk factors of first diagnosed T2DM with MS through collected my courtyard January,2000 to the May,2008 first diagnosed T2DM correlation data.Results:1.The prevalence rate of first diagnosed T2DM with MS was 62.8%.and was negatively correlated with age,and positively with physique index,women with Obese production history and Family history.⒉The prevalence rate of first diagnosed T2DM with hypertension was 51.7%.⒊ The proportion of first diagnosed T2DM Physique index ≥25.0 kg/m2 was 63.2%.⒋ There were 69.9% patients with Dyslipidemia in first diagnosed T2DM with Dyslipidemia.⒌ The prevalence rate of women first diagnosed T2DM with Obese production history with MS was 69.3%.⒍ The prevalence rate of first diagnosed T2DM with MS who have MS family history was 72.4%.Conclusions:⒈ The prevalence rate of first diagnosed T2DM with MS was 62.8%,and was negatively correlated with age,and positively with women with Obese production history and MS Family history.⒉ Early weight control,particularly who with family history of MS is the key of preventing first diagnosed T2DM.