Current situation and utilization of informatization construction with faculty's health files in colleges and universities in Yanta district of Xi'an city of Shanxi Province / 中国基层医药

Objective To analyze the problem existing in the process of health records informatization con-struction and explore solution on the basis of situation of health record informatization in Yanta district of Xi'an city. And to provide bases for acceleration of residents'health records informatization construction in Xi'an.Methods Send 10 investigators to study and investigate the hospital information management system of 10 full-time public universities in Yanta district.Results There were a total of 25 711 faculties in 10 full-time public universities and 19 809 facul-ties had established electronic files,the filing rate was 77.04% A.On the whole,it seemed that the teaching staff in the filing rate(78.91%)was higher than that of the retired workers filing rate(73.78%),the two groups had statisti-cally significant difference(χ2 =88.164,P <0.05)B.Conclusion Xi'an Yanta district university faculty of electron-ic health records has exceeded the national average level,but there are still many problems need to be improved.We should strengthen government investment,the establishment of the region's unified information management standards, increase publicity and education efforts,and actively create the region's resource information sharing platform.

Effect of Pepper and Piper Longum on Hepatic Energy Metabolism Factors in Rats / 世界科学技术-中医药现代化

This study was aimed to explore the effect of heat-nature Chinese herbs of Pepper and Piper longum on hepatic energy metabolism of rats in order to research the impact of ratˊs physical activities with heat-nature Chinese herbs and establish the research foundation of Chinese herbal medicine nature . A total of 30 rats were randomly divided into three groups . Saline was given in the control group . Pepper water extract was given in the Pepper group . And Piper longum water extract was given in the Piper longum group . The administration dosage was 10 ml/kg intragastrically for 30 days . Changes of hepatic energy metabolism related enzymes were determined . The results showed that compared with the control group , Ca2+-ATPase , Ca2+-Mg2+-ATPase , and LDH enzyme of the Pepper group and Piper longum group were significantly increased . And the hepatic glycogen content was significantly reduced . It can obviously increase energy intake , digestible energy and metabolizable energy per body weight in rats . There was no statistical significance between the Pepper group and Piper longum group . It was concluded that the heat-nature Chinese herbs of Pepper and Piper longum can promote the decomposition of hepatic glycogen , increase the activity of LDH enzyme , Ca2+-ATPase , Ca2+-Mg2+-ATPase . It increases the consumption of ATPase in order to increase energy intake , digestible ener-gy and metabolizable energy per body weight in rats and promote the hepatic energy metabolism .

Induction of Angiogenesis and Neurogenesis by Serum from Rats Treated with Shunaoxin Dropping Pills / 中草药·英文版

Objective Shunaoxin Dropping Pills (SDPs), a Chinese patent medicine, has been used widely in China for the treatment of headache, amnesia, and insomnia. The aim of the present study is to observe the effect of SDPs on inducing angiogenesis and neurogenesis in vitro. Methods The present testing system using the serum obtained from animals ig treated with SDPs and a co-culture system in vitro was used to investigate if SDPs promotes brain microvascular endothelial cells (BMECs) tube formation and neural differentiation of neural stem/progenitor cells (NSPCs), which plays important roles in angiogenesis and neurogenesis. Results The SDPs serum sampled from rats ig treated with SDPs for 3 d dose-dependently promoted the tube like structure formation of cultured BMECs, and enhanced the fraction of MAP-2 positive cells of NSPCs, which co-cultured with the BMECs and astrocyte. In addition, there was no significant change in the percentage of glial fibrillary acidic protein positive cells. Conclusion Our results show that SDPs serum can induce neural differentiation and BMECs tube formation in vitro.

Mechanism of protective effect of pretreatment of paeoniflorin on injured cortical neurons with corticosterone / 中国中药杂志

<p><b>OBJECTIVE</b>Using the method of reverse transcription-polymerase chain reaction (RT-PCR) to detect the related gene expression,with a view to clarify the principal mechanism that the neuroprotection of paeoniflorin (PF).</p><p><b>METHOD</b>At the seventh day, after the pretreatment of PF (0.5, 2, 10 micromol x L(-1)), with 30 min primary cultures of neurons from the cerebral cortex of 16 days old embryo rats were injured with corticosterone (200 micromol x L(-1)). After 48 h, the methods of RT-PCR was used to detect the expression of Bcl-2, Bax, Caspase-3, BDNF mRNA.</p><p><b>RESULT</b>Compared with model group, the dose of PF(2, 10 micromol x L(-1)) not only can degrade the expression of Bax, Caspase-3 mRNA, but also can raise the expression of Bcl-2, BDNF mRNA notablely (P < 0.05).</p><p><b>CONCLUSION</b>The data indicated that the principal mechanism of prophylactic neuroprotective effect of PF on corticosterone-induced neurons damages maybe due to PF can raise the expression of apoptosis-related genes Bel-2, Bax, Caspase-3 mRNA, and also ascribe to PF can raise the expression of neurotrophic factor.</p>

Prophylactic protective effects of paeoniflorin on corticosterone-induced primary cultures of rat cortical neurons damages / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the prophylactic neuroprotective effect of paeoniflorin (PF) on corticosterone-induced primary cultures of rat cortical neurons damages.</p><p><b>METHOD</b>Primary cultures of neurons from the of 16-days-old embryo rats were used to study. Neurons in the experimental group were treated with cort (200 micromol x L(-1)) 30 min after the pretreatment of PF (0.5, 2, 10 micromol x L(-1)). We used MTT assay to detect the action of paeoniflorin on neuronal activity. DAPI staining to study of the influence of PF on neuronal apoptosis morphology, and Annexin V-FITC/PI apoptosis detection kit to inspect the impact of paeoniflorin on the early neuronal apoptosis.</p><p><b>RESULT</b>Compared with model group, every dose of PF (0.5, 2, 10 micromol x L(-1)) can raise the neuronal activity notably (P<0.05), PF of 2 micromol x L(-1) and 10 micromol x L(-1) can reduce the rate of neuronal apoptosis.</p><p><b>CONCLUSION</b>Our data indicated that PF achieved prophylactic neuroprotective effect through against corticosterone-induced neurons damages.</p>

The effect of Tanshinone IIA on the expression of NF-?B、I?B-? and the mRNA of ICAM-1 and VCAM-1 of ECV304 induced by TNF-? / 中国药理学通报

Aim To illustrate the role of Tanshinone IIA(TanIIA)extracted from dan-shen root in atherosclerosis and its different targets by investigating the effect on the expression of NF-?B、I?B-? and the mRNA of ICAM-1 and VCAM-1 in human umbilical vascular endothelial cell 304(ECV304).Mehods The TNF-?-induced cultured ECV-304 cell injury model was established to analyze the expression of NF-?B、I?B-? by cell ELISA method and the mRNA of ICAM-1 and VCAM-1 by RT-PCR method of the cells cultured with different denses of TanIIA.Results The results of ELISA method showed that the expression of the NF-?B was reduced significantly and the expression of the I?B-? was enhanced significantly in ECV304 which were cultured with higher denses of TanIIA.The results of RT-PCR method showed that the mRNA of ICAM-1 and VCAM-1 induced by TNF-? was significantly refrained.Conclusions TanIIA has a significant effect on inhibiting the mRNA of ICAM-1 and VCAM-1 induced by the NF-?B activation,so it can play an impotant role in resisting atherosclerosis.