ABSTRACT
Objective:To explore influence of diltiazem hydrochloride on expression of serum microRNA in aged pa‐tients with dilated cardiomyopathy (DCM ) . Methods :A total of 120 aged DCM patients (DCM group ) and 60 healthy aged people (normal control group) were enrolled .DCM group was further randomly and equally divided in‐to routine treatment group (n=60) and diltiazem group (n=60 ,received additional diltiazem hydrochloride based on routine treatment) .Left ventricular end -diastolic dimension (LVEDd) ,left ventricular posterior wall thickness (LVPWT) and left ventricular ejection fraction (LVEF) were observed and compared between routine treatment group and diltiazem group before and after treatment ,cardiac function improvement was compared between these two groups after treatment .Changes of expression amount of serum miR -1 ,-133 ,-499 and -208 were com‐pared among three groups before ,six and 12 weeks after treatment .Results:Compared with before treatment ,after treatment ,there were no significant changes in LVEDd ,LVPWT and LVEF in routine treatment group , P>0.05 all;there were significant reductions in LVEDd [(69.41 ± 6.42) mm vs .(61.32 ± 5.31) mm] and LVPWT [(20.98 ± 1.98) mm vs .(12.05 ± 2.42) mm] and significant rise in LVEF [(32.81 ± 4.71)% vs .(50.52 ± 6.88)% ] in dilt‐iazem group , and LVEDd , LVPWT were significantly lower than those of routine treatment group [LVEDd:(67.25 ± 4.21) mm ,LVPWT :(18.49 ± 2.86) mm] ,and LVEF was significantly higher than that of routine treat‐ment group [ (47.77 ± 3.92)% ] , P<0.05 all .For cardiac function improvement ,markedly effective rate (71.7%vs .56.7% ) and total effective rate (98.3% vs .85.0% ) of diltiazem group were significantly higher than those of routine treatment group , P<0.05 both . Compared with normal control group , the 4 kinds of miR expression sig‐nificantly reduced in routine treatment group , and compared with routine treatment group , those of diltiazem group were significantly rose , P<0.01 all , but compared with 6‐week , miR expression had downtrend during 12‐week , the miR‐208 expression was most significant ( P< 0.01 ) .Conclusion:Diltiazem could effectively modulate serum microRNA expression in aged CDM patients ,which provided molecular biological basis for studying diltiazem mech‐anism .