ABSTRACT
【Objective】 To analyze the expression and clinical significance of CD4+ CD69+ T cells in peripheral blood of patients with autoimmune hemolytic anemia. 【Methods】 A total of 206 cases of autoimmune hemolytic anemia admitted to our hospital from March 2018 to March 2021 were selected as the observation group, and 206 cases, who came for healthy physical examination during the same period, were selected as the control group. The levels of CD4+ CD69+ T cells in peripheral blood, CD69 mRNA in CD4+ T cells and CD69 in plasma were detected. The relationship between CD4+ CD69+ T cell level and Hb, reticulocyte ratio (Ret), total bilirubin (TBIL) and indirect bilirubin (IBIL) was analyzed by Pearson correlation analysis to observe the occurrence of venous thromboembolism. The AUC of receiver operating characteristic was used to evaluate the predictive efficacy of CD4+ CD69+ T cell expression in peripheral blood for venous thromboembolism. 【Results】 The levels of CD4+ CD69+ T cells in peripheral blood, CD69 mRNA in CD4+ T cells and CD69 in plasma of observation group were significantly higher than those of control group (P<0.05). Among 206 patients in the observation group, the levels of CD4+ CD69+ T cells in peripheral blood, CD69 mRNA in CD4+ T cells and plasma CD69 in hemolytic attack group were significantly higher than those in remission group, with statistical significance(P<0.05). The Hb in hemolytic attack group was significantly lower while Ret, TBIL and IBIL levels were significantly higher than those in remission group, with statistical significance(P<0.05). Pearson correlation analysis showed that the levels of CD4+ CD69+ T cells in peripheral blood of patients with autoimmune hemolytic anemia were negatively correlated with Hb(P<0.05) while positively correlated with Ret, TBIL and IBIL(P<0.05). According to ROC curve analysis, the expression level of CD4+ CD69+ T cells in peripheral blood predicted that the AUC of patients with autoimmune hemolytic anemia complicated with venous thromboembolism was 0.915(SE: 0.068, OR: 0.002, 95%CI: 0.000~1.000). 【Conclusion】 The up-regulated expression level of CD4+ CD69+ T cells in peripheral blood of patients with autoimmune hemolytic anemia is closely related to disease evolution, and it has good efficacy in predicting venous thromboembolism and deserves clinical attention.