ABSTRACT
In vitro culture of the mutualistic fungus of leaf-cutting ants is troublesome due to its low growth rate, which leads to storage problems and contaminants accumulation. This paper aims at comparing the radial growth rate of the mutualistic fungus of Atta sexdens rubropilosa Forel in two different culture media (Pagnocca B and MEA LP). Although total MEA LP radial growth was greater all along the bioassay, no significant difference was detected between growth efficiencies of the two media. Previous evidences of low growth rate for this fungus were confirmed. Since these data cannot point greater efficiency of one culture medium over the other, MEA LP medium is indicated for in vitro studies with this mutualistic fungus due its simpler composition and translucent color, making the analysis easier.
Subject(s)
Animals , Agaricus procerus , Ants , Culture Media , Fungi/growth & development , In Vitro Techniques , Mycelium/growth & development , Methods , MethodsABSTRACT
Feito um levantamento de 61 casos de tumores renais em adultos, ocorrido no periodo de 1964 a 1981, ressaltando a importancia de estudo clinico e radiologico na feitura do diagnostico e mostrando as dificuldades encontradas para a feitura precoce desse diagnostico, o que leva a obtencao de resultados aquem dos desejados apos a instituicao do tratamento