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Aim To investigate the protective effect of baicalin on renal fibrosis in rats with diabetic nephrop- III (Col- III) athy (DN) and to investigate its mechanism of action. Methods A rat model of diabetic nephropathy was constructed. The rats were randomly divided into control group, model group, baicalin low dose group, baicalin medium dose group, baicalin high dose group and metformin group, with 10 rats in each group. Except for the control group, all rats in each group were fed with streptozotocin 65 mg • kg -
ABSTRACT
Objective: To explore the extraction process of Trollii Flos Oral Liquid (TFOL). Methods: HPLC was used to determine orientin and vitexin in liquid extract of TFOL, using mass concentration of both as index. The orthogonal design L18(37) and reverse continuous cycle cryogenic extraction were used to extract Trollii Flos, the optimum extraction was determined, compared with the current standard of TFOL extraction process. Results: The optimum extraction conditions of reverse continuous cycle cryogenic extraction were as follows: extraction temperature 90°C, extraction time 2 h, powder type 10 mesh, added of 20 times water. The linear ranges of orientin and vitexin were 18.46-295.37 (r = 0.9999) and 5.84-93.44 μg/mL (r = 0.9999), the average recoveries of orientin and vitexin were 96.07%, RSD = 0.74% (n = 6), and 97.73%, RSD = 1.42% (n = 6), respectively. Conclusion: The technology of reverse continuous cycle cryogenic extraction is stable and feasible, and the extraction rate is high; The measurement method is accurate, sensitive, simple, and stability, and has good reproducibility. The extraction and determination methods could be used as production process and quality control reference of TFOL.