ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of puerarin on ADRP gene mRNA expression in fatty tissue of type 2 diabetes mellitus rats (T2DM).</p><p><b>METHOD</b>Wiastar rats of T2DM model were made by feeding with high glucose and fat diet and injecting with small dose of streptozocin (25 mg x kg(-1)). 40 model rats were randomly divided into model control group and three puerarin groups (40, 80, 160 mg x kg(-1)), another 10 rats were selected as normal control group. FBG and FINS were measured to calculate IR after rats were injected consecutively for 6 weeks. The level of ADRP gene mRNA in fatty tissue was determined by RT-PCR after rats were injected eight weeks.</p><p><b>RESULT</b>Compared with model control group, high and middle dosage of puerarin can decreased ADRP gene mRNA expression in fatty tissue obviously, FBG, IR level in each puerarin group and FINS in high and middle dosage puerarin groups decreased obviously.</p><p><b>CONCLUSION</b>Puerarin can decrease the blood glucose level of T2DM by downregulating ADRP mRNA expression and depressing the insulin resistance.</p>
Subject(s)
Animals , Female , Male , Rats , Adipose Tissue , Metabolism , Blood Glucose , Diabetes Mellitus, Experimental , Drug Therapy , Genetics , Gene Expression Regulation , In Vitro Techniques , Isoflavones , Pharmacology , Membrane Proteins , Genetics , Perilipin-2 , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Vasodilator Agents , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To evaluate the clinical therapeutic effect of electric acupuncture (EA) at Sishencong (EX-HN 1) on insomnia.</p><p><b>METHODS</b>Two hundred and seventy-six patients were randomly assigned to 2 groups, 138 in each group, the EA group treated with EA at Sishencong, and the control group with oral administration of Tianmeng Capsule. The treatment course for both groups was 3 weeks. The quality and related parameters of sleep before and after treatment were evaluated with a multi-channel sleep detector.</p><p><b>RESULTS</b>After treatment, the quality of sleep was improved in both groups (P < 0.05), as compared with before treatment, the difference in related parameters was significant respectively (P<0.05 or P <0.01), however, the improvement in the EA group was superior to that in the control group (P < 0.01).</p><p><b>CONCLUSION</b>EA at Sishencong has obvious effect on insomnia.</p>