ABSTRACT
AIM To study the chemical constituents from Pogonatherum crinitum (Thunb.) Kunthand and their in vitro anti-HBV activities.METHODS The ethyl acetate and n-butanol fractions of 70％ ethanol extract from P.crinitum were isolated and purified by AB-8 macroporous resin,silica column and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The in vitro anti-HBV activities of the isolated compounds were tested in the model of HepG2.2.15 cells.RESULTS Eight compounds were isolated and identified as 6,8,4'-trihydroxy-7,3'-dimethoxyisoflavone (1),quercetin-7,4'-dimethylester-5-O-β-D-glucopyranoside (2),8-(1-(3,4-dihydroxyphenyl) ethyl) quercetin (3),chrysoeiml-7-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranoside (4),kaempferol-3-O-(2",3"-di-O-E-p-cou-maroyl)-α-L-rhamnopyranoside (5),1,3,7-trihydroxyxanthone-2-C-β-D-glucopyra-noside (6),luteolin-6-C-β-D-boivinopyranoside (7),luteolin-6-C-β-D-glucopyranoside (8).Compounds 3,5,7,8 had certain inhibitory effects on HBsAg with the IC50 values of 96.6,127.1,72.4 and 54.5 μg/mL.Compounds 7,8 had certain inhibitory effects on HBeAg with the IC50 values of 81.0,61.3 μg/mL.CONCLUSION Compounds 1-6 are isolated from genus Pogonatherum for the first time,compounds 3、5、7、8 have certain in vitro anti-HBV activities.
ABSTRACT
Objective: To evaluate the effect of 8-epi-kingiside (8-Epik) derived from the buds of Jasminum officinale var. grandiflorum (JOG) on hepatitis B virus (HBV) replication in HepG2 2.2.15 cell line in vitro and duck hepatitis B virus (DHBV) replication in ducklings in vivo. Methods: The concentration of extracellular hepatitis B e antigen and hepatitis B surface antigen (HBsAg) in cell culture medium was determined by ELISA, respectively. The anti-HBV effects of 8-Epik were also demonstrated in the model of DHBV. 8-Epik was ip given (20, 40, and 80 mg/kg, twice daily) to the DHBV-infected ducklings for 10 d. The isotonic saline liquid diet was ip given as negative control and Lamivudine (50 mg/kg, twice daily) was given as positive control. DHBV DNA was measured at days 0 (T0), 5 (T5), 10 (T10), and day 3 after cessation of treatment (P3) by dot blotting. Results: 8-Epik effectively blocked HBsAg secretion in HepG2 2.2.15 cells in a dose-dependent manner [IC50 = (19.4 ± 1.04) μg/mL]. 8-Epik (40 or 80 mg/kg, ip, twice daily) also reduced viremia in DHBV-infected ducks. Conclusion: Therefore, 8-Epik is warranted as a potential therapeutic agent for HBV infection. © 2013 Tianjin Press of Chinese Herbal Medicines.
ABSTRACT
The study on the buds of Jasminum officinale L. var. grandiflorum was carried out to look for anti-HBV constituents. The isolation and purification were performed by HPLC and chromatography on silica gel, polyamide and Sephadex LH-20 column. The structures were elucidated on the basis of physicochemical properties and spectral analysis. Six iridoid glycosides were identified as jasgranoside B (1), 6-O-methy-catalpol (2), deacetyl asperulosidic acid (3), aucubin (4), 8-dehydroxy shanzhiside (5), and loganin (6). Jasgranoside B (1) is a new compound. Compounds 2-6 were isolated from Jasminum officinale L. var. grandiflorum for the first time.
Subject(s)
Humans , Antiviral Agents , Chemistry , Pharmacology , Flowers , Chemistry , Glucosides , Chemistry , Pharmacology , Hep G2 Cells , Hepatitis B Surface Antigens , Metabolism , Hepatitis B virus , Allergy and Immunology , Inhibitory Concentration 50 , Iridoid Glucosides , Chemistry , Iridoid Glycosides , Chemistry , Pharmacology , Iridoids , Chemistry , Jasminum , Chemistry , Molecular Structure , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituent bud of the flowers of Jasminum officinale var. grandiflorum.</p><p><b>METHOD</b>The compounds were isolated and purified by recrystallization and chromatography on silica gel and Sephadex LH - 20 column. Their structures were elucidated on the basis of physicochemical properties and spectral analysis.</p><p><b>RESULT</b>Six triterpenoid saponins were identified as 3-O-alpha-L-rhamnopyranosyl (1 --> 2)-beta-D-xylopyranosyl- hederagenin-28-O-beta-D-galactopyranosyl (1 --> 6)-beta-D-galactopyranosyl ester (1), hederagenin-3-O-beta-D-glucopyranosyl (1 --> 3)-alpha-L-arabinopyranoside (2), 2alpha, 3beta, 23-trihydroxyolean-12-en-28-oic-O-beta-D-glucopyranosyl ester (3), hederagenin-3-O-beta-D-xylopyranosyl (1 --> 3)-alpha-L-rhamnopyranosyl (1 --> 2)-alpha-L-arabinopyranoside (4), 2alpha, 3beta, 23-trihydroxyolean-12-en-28-oic-O-alpha-L-rhamnopyranosyl (1 --> 4)-beta-D-glucopyranosyl (1 --> 6)-beta-D-glucopyranosyl ester (5), hederagenin-3-O-alpha-L-rhamnopyranosyl (1 --> 2)-alpha-L-arabinopyranoside (6).</p><p><b>CONCLUSION</b>Compound 1 is a new compound. Compounds 2, 3, 4, 5, 6 were isolated from the genus Jasminum for the first time.</p>
Subject(s)
Chromatography, Gel , Drugs, Chinese Herbal , Chemistry , Flowers , Chemistry , Jasminum , Chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Saponins , Chemistry , Triterpenes , ChemistryABSTRACT
To study the chemical constituents of the flowers of Jasminum officinale L. var. grandiflorum, the compounds were isolated and purified by HPLC, recrystallization and chromatography on silica gel and Sephadex LH-20 column. Their structures were elucidated on the basis of physicochemical properties and spectral analysis. Six secoiridoids were identified as jasgranoside (I), jaspolyoside (II), 8-epi-kingiside (III), 10-hydroxy-oleuropein (IV), 10-hydroxy-ligstroside (V), oleoside-7, 11-dimethyl ester (VI). Compound I is a new compound. Compounds II, III, IV, V and VI were isolated from Jasminum officinale L. var. grandiflorum for the first time.
Subject(s)
Flowers , Chemistry , Glucosides , Chemistry , Guaiacol , Chemistry , Iridoid Glucosides , Iridoids , Chemistry , Jasminum , Chemistry , Molecular Structure , Plants, Medicinal , ChemistryABSTRACT
To study the chemical constituents of the flower of Jasminum officinale L. var. grandiflorum. The compounds were isolated and purified by re-crystallization and chromatography on silica gel and Sephadex LH-20 column. Their structures were elucidated on the physicochemical properties and spectral analysis. Seven glycosides were identified as kaempferol-3-O-alpha-L-rhamnopyranosyl (1-->3)-[alpha-L-rhamnopyranosyl (1-->6)]-beta-D-galactopyranoside (I), kaempferol-3-O-rutinoside (II), 7-ketologanin (III), oleoside-11-methyl ester (IV), 7-glucosyl-l1-methyl oleoside (V), ligstroside (VI), oleuropein (VII). Compound I is a new compound. Compounds III and V were isolated from the family of Jasminum for the first time and compounds II, IV and VI were isolated from Jasminum officinale L. var. grandiflorum for the first time.