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Objective To investigate the effects of soybean peptide calcium on apparent calcium absorption rate and bone mineral density of rats.Methods Totally 50 male Wistar rats were divided into 5 groups at comparable body weight:the low calcium control group,calcium carbonate control group and 3 groups with low,medium and high dose of soybean peptide calcium(SPC).Rats of each group were fed with designed forage.At the end of the fourth week of the feeding.calcium metabolic experiment was performed for 3 days and the apparent calcium absorption rate was calculated.Three months later,the left and right femurs of the rats were stripped to assay the bone calcium and bone density.Results Apparent calcium absorption rate of the calcium carbonate control group was 69.9 ％,which was lower than medium(82.6 ％) and high doses(77.4％) of soybean peptide calcium groups(F =31.371,P=0.000,0.001).Compared with the low calcium control group,rats with medium and high doses of SPC had obviously increased levels of weights(F =3.150,P =0.010,0.002),bone mass(F =3.195,P =0.015,0.002),calcium content (F=4.506,P =0.004,0.001)and bone mineral density of midpoint (F=6.185,P =0.006,0.000) and far end of femur (F =6.081,P=0.027,0.000).Despite of the weight index (P＞0.05),the high dose of soybean peptide calcium group also had the same results as the calcium carbonate control group (P=0.046,0.024,0.013,0.001).Conclusions Soybean peptide calcium can promote the apparent calcium absorption rate and increase the bone mineral density.
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Objective To investigate dietary fat intake and fatty acid composition in Qingdao residents.Methods Cluster random sampling method was used to select 189 adults (53 males and 136 females) between March and to July 2010.The food intake habit and the relationship between nutrient,fat,fatty acid composition and serum lipid concentrations were analyzed.Results Per capita intake of cereal was 200 g,potato 26 g,poultry meat and offal 85 g,seafood 73 g,nuts 23 g,and oils 40 g.Protein accounted for 16％ of total calorie intake,carbohydrate 48％,and fat 37％.Oils accounted for 43％ of fat intake,and nuts 11％.High fat intake was mainly contributed to intake of oils and nuts.Those with dyslipidemia showed higher nutritent intake and body mass index.ConclusionQingdao residents show higher dietary fat intake and lower long-chainω-3 (n-3) PUFAs consumption.Long-chain n-3 PUFAs may be negatively correlated with dyslipidemia.
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Objective: To study the inhibition and mechanism of tea polyphenols (TP) on apoptosis induced by oxidized low density lipoprotein (ox-LDL) in human umbilical vein endothelial cells (HUVEC). Method: HUVEC cells were administered with TP (25 ?g/ml), ox-LDL (200 ?g/ml)+TP (25 ?g/ml), ox-LDL (200 ?g/ml) respectively, cells with equal volume of solvent as control. Cell viability was determined by MTT assay, apoptosis by acridine orange fluorescence dyeing, and expressions of Bcl-2、Bax and caspase-3 were analyzed by Western blotting. Results: Ox-LDL could inhibit HUVEC cell proliferation. After treated with both TP and ox-LDL , the cell proliferation increased obviously and showed significant difference from the cells treated with ox-LDL only (P
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Objective:To determinate the resistant starch in different kinds of food so as to provide the data for guiding the diet for health. Method:The contents of resistant starch in 6 kinds of food samples were determined by a method that mimicked gastrointestinal conditions. Results:The contents of resistant starch in raw food were higher than cooked food, except beans. The content of resistant starch of raw potato and deepfrying potato was respectively the highest, 46.06?2.65 g/100g total starch and 29.11?1.52 g/100g total starch. Conclusion:The repeatability of the analysis method was good, and the contents of resistant starch in various kinds of food were different.
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Objective: To investigate the relationship between serum leptin concentration and diet nutrition, age and gender. Method: One hundred fifty-eight men and women aged 18-72 y were initially drawn from a community in Jinan City. The parameters measured included height, weight and serum leptin concentrations. In addition, a 3-d food record was used to represent usual food intake by the participants. Results: The mean serum leptin concentration in males was 8.38?6.31ng/ml, while in females 24.98?13.29 ng/ml. Serum leptin level in males was significantly correlated with age, but not in females. Respectively, serum leptin level in males and females were not correlated to dietary factors. After adjusted for BMI, serun leptin level in females was positively correlated with dietary intake of fat, MUFA and PUFA. Conclusion: Serum leptin level in female is higher than that in male. Age is correlated to serum leptin level in males. The relation of dietary intake of fat, MUFA and PUFA to serum leptin concentration only existed in women.
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Objective: To investigate the antiatherosclerotic effect of the extract from grape seed in C57BL/6J mice. Method: The mice were fed with atherosclerotic diet for 20 w and then with basic diet or different dosage of grape seed extract for 4 w. The blood samples were examined for blood lipid. The sections of aorta were examined and scored microscopically. Results: After fed with atherogenic diet for 20 w, the aortic score was 7.2?1.3 and TG, Chol and MDA in serum were significantly increased. After fed with the extract of grape seed for 4 w, the aortic score was reduced to 6.0?1.2, and TG, TC, and MDA levels also decreased. Conclusion: The extract of grape seed has antiatherosclerotic effect.