ABSTRACT
To investigate the effects of lidocaine on phospholipase A_2(PLA_2) and fluidity of lung cell membrane during rabbits endotoxemia. Method: Twenty-four white rabbits were randomly assigned to receiving one of three treatmemnts (n=8 for each group): infusion of saline(control group), infusion of Escbericbia coli endotoxin(endotoxin group), infusion of endotoxin with lidocaine pretreatment (lidocaine group). The PLA_2 activity, PS, PE and PC contents of lung cell membrane fluidity were analysed with high performance liquid chromatography and DPH method respectively. Result: PLA_2 activities in the plasma and cell membrane were reduced significantly, and the PS, PE and PC contents in the cell membrane increased significantly in lidocaine group compared with those in endotoxin group. The membrane fluidities of RBC and lung cells of rabbit with lidocaine pretreatment also incresed significantly in comparison with those in endotoxin group. Conclusion:The pretreatment with lidocaine inhibits PLA_2 activity in plasma and cell membrane of rabbit lung, reduces the contents of phospholipids in cell membrane of rabbit lung, increases membrane fluidity of RBC and lung ceils of rabbit and stabilizes cell membranes function.