ABSTRACT
Abstract Lichens have exhibited numerous biological activities, including growth inhibition of tumor cells. This study evaluated the antiproliferative activity of hypostictic and salazinic acids against tumor cell lines (B16-F10, PC-03, MCF7, HT-29, HEP-G2, K562 and 786-0) by the SRB assay in vitro and antitumor activity in experimental murine melanoma in vivo. Activation of caspase-3 was quantified by flow cytometry. The murine experimental melanoma model B16-F10 was used in BALB/c mice for evaluation of antitumor activity. Hypostictic acid showed significant antiproliferative activity in K562 cells (GI50 2.20 µM), B16-F10 (GI50 13.78 µM) and 786-0 (GI50 14.24 µM), whereas salazinic acid was more active against K562 cells (GI50 64.36 µM), HT-29 (GI50 67.91 µM) and B16-F10 (GI5078.64 µM). Quantification of capase-3 revealed that the test compounds did not increase the expression of that enzyme. In the in vivo antitumor evaluation in B16-F10 melanoma, the isolated compounds inhibited tumor growth in relation to weight and volume. Hypostictic acid (16.7 mg/kg) inhibited 72% and salazinic acid 88% of tumor volume (p < 0.05). The results indicated that, both in the in vitro and in vivo models, the compounds evaluated showed antiproliferative and antitumor activities.
ABSTRACT
ABSTRACT PURPOSE: To perform an integrative review of the main animal disease models in rodents used for obesity. METHODS: Research was conducted in the CAPES Portal database using the following keywords "obesity animal models, diet and rodents", published between the years 2010 to 2016. We found 108 articles, of which 19 were selected and analyzed in full for this study. RESULTS: Larger part of publications occurred in the last 6 years, the rats (n = 10) were used in the same proportion mice (n = 10). The choice of male animals (n = 18) and age greater than 21 days (n = 17) showed a major highlight. The greater than 5 week follow-up period (n = 18) was the most applied. A High Fat Diet was the most used in studies (n = 18) . CONCLUSIONS: Male rodents continue to be considered the species most used in experimental studies to induce obesity, also was found variations of age to the beginning of the experiment. For the most part are follow-up time studies along with the use of High Fat Diet.
Subject(s)
Animals , Male , Female , Mice , Rats , Rodentia , Animal Experimentation/statistics & numerical data , Diet , Disease Models, Animal , Obesity/physiopathology , Diet, High-FatABSTRACT
Os óleos de frutos estão sendo estudados por seu potencial na indústria de alimentos e também para aumento do consumo de ácidos graxos monos e poli-insaturados. Este estudo objetivou determinar as constantes físico-químicas, composição centesimal, identificar e quantificar os principais ácidos graxos presentes na fração lipídica da polpa e da amêndoa dos frutos de guarirova, espécie Syagrus oleracea (Mart.) Becc. Foi realizada a caracterização físico-química dos óleos, composição de ácidos graxos e índices de qualidade nutricional. O óleo da amêndoa possui maior concentração de ácidos graxos saturados (89,2%), sendo representado principalmente pelo ácido láurico (48,34%). A polpa apresentou ácidos graxos monoinsaturados (24,84%) e poli-insaturados (33,25%), com maior percentual de ômega-6 (31,94%) e ômega-9 (19,15%); e menor de ômega-3 (1,31%).
The oils of fruits are being studied for its potential in the food industry and also to increase the consumption of mono and polyunsaturated fatty acids. This study aimed to determine the physio-chemical constants, chemical composition and identify and quantify the major fatty acids present in the lipid fraction of pulp and kernel of the fruits of guarirova, species Syagrus oleracea (Mart.) Becc. It was performed the physicochemical characterization of oils, fatty acid composition and nutritional quality indexes. Almond oil has the highest concentration of saturated fatty acids (89,2%), represented mainly by lauric acid (48,34%). The pulp showed monounsaturated (24,84%) and polyunsaturated fatty acids (33,25%), with higher percentage of omega-6 (31,94%) and omega-9 (19,15%) and lower omega-3 (1,31%).