ABSTRACT
PURPOSE: To evaluate the relative gene expression (RGE) of cytosolic (MDH1) and mitochondrial (MDH2) malate dehydrogenases enzymes in partially hepatectomized rats after glutamine (GLN) or ornithine alpha-ketoglutarate (OKG) suplementation. METHODS: One-hundred and eight male Wistar rats were randomly distributed into six groups (n=18): CCaL, GLNL and OKGL and fed calcium caseinate (CCa), GLN and OKG, 0.5g/Kg by gavage, 30 minutes before laparotomy. CCaH, GLNH and OKGH groups were likewise fed 30 minutes before 70% partial hepatectomy. Blood and liver samples were collected three, seven and 14 days after laparotomy/hepatectomy for quantification of MDH1/MDH2 enzymes using the real-time polymerase chain reaction (PCR) methodology. Relative enzymes expression was calculated by the 2-ΔΔC T method using the threshold cycle (CT) value for normalization. RESULTS: MDH1/MDH2 RGE was not different in hepatectomized rats treated with OKG compared to rats treated with CCa. However, MDH1/MDH2 RGE was greater on days 3 (321:1/26.48:1) and 7 (2.12:1/2.48:1) while MDH2 RGE was greater on day 14 (7.79:1) in hepatectomized rats treated with GLN compared to control animals. CONCLUSION: Glutamine has beneficial effects in liver regeneration in rats by promoting an up-regulation of the MDH1 and MDH2 relative gene expression. .
Subject(s)
Animals , Male , Gene Expression/drug effects , Glutamine/pharmacology , Hepatectomy/methods , Liver Regeneration/drug effects , Malate Dehydrogenase/metabolism , Ornithine/analogs & derivatives , Liver Regeneration/physiology , Models, Animal , Malate Dehydrogenase/genetics , Ornithine/pharmacology , Random Allocation , Rats, Wistar , Real-Time Polymerase Chain Reaction , Reference Values , Reproducibility of Results , Time Factors , Up-RegulationABSTRACT
A doença de Caroli é uma má-formação congênita rara caracterizada por dilatações multifocais dos ductos biliares intra-hepáticos que predispõem a colestase e episódios recorrentes de colangite. Descrevemos o caso de um paciente jovem diagnosticado com doença de Caroli, por meio da colangiorressonância, que complicou com abscesso hepático e foi submetido a drenagem cirúrgica.
Caroli's disease is a rare congenital malformation characterized by multifocal dilatation of intrahepatic bile ducts predisposing to cholestasis and recurrent cholangitis. The authors describe the case of a young patient with Caroli's disease diagnosed by magnetic resonance cholangiography and complicated with liver abscess, being submitted to surgical drainage.
Subject(s)
Humans , Male , Adolescent , Congenital Abnormalities , Caroli Disease/diagnosis , Bile Ducts/pathology , Cholangiography , Magnetic Resonance Spectroscopy , UltrasonographyABSTRACT
Mucocele is an unusual lesion of the appendix characterized by accumulation of mucoid substance inside the appendix, which occurs as a sequel of luminal obstruction, usually by fibrous tissue. Diagnosis is intraoperative in most of the cases. We discuss the clinical, anatomicopathological and therapeutics aspects of the disease.
ABSTRACT
We report the case of a one-year and two-months-old child with a choledochal cyst tYpe I of Alonso-Lej and Todani's classification , diagnosed through abdominal ultrasound. The surgical treatment was cholecistectomy and choledochal cyst excision with Roux-in-Y hepatic- jejunostomy. Minor complications were observed during early postoperative recovery. Long-term flow-up has been uneventiful, with overall improvement.